Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Other assay [5]
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Validation data
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- Product number
- MA1-772 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MMP2 Monoclonal Antibody (F14 P4 D3)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA1-772 contains 100 µg of in vitro produced, protein A purified antibody (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide.
- Antibody clone number
- F14 P4 D3
- Concentration
- 1 mg/mL
Submitted references Embryonic Trophectoderm Secretomics Reveals Chemotactic Migration and Intercellular Communication of Endometrial and Circulating MSCs in Embryonic Implantation.
miR‑205 suppresses cell migration, invasion and EMT of colon cancer by targeting mouse double minute 4.
Silence of Stomatin-Like Protein 2 Represses Migration and Invasion Ability of Human Liver Cancer Cells via Inhibiting the Nuclear Factor Kappa B (NF-κB) Pathway.
Forkhead box C2 promotes the invasion ability of human trophoblast cells through Hedgehog (Hh) signaling pathway.
MicroRNA-27a Inhibits Cell Migration and Invasion of Fibroblast-Like Synoviocytes by Targeting Follistatin-Like Protein 1 in Rheumatoid Arthritis.
In vitro assays of rod and cone opsin activity: retinoid analogs as agonists and inverse agonists.
Calle A, Toribio V, Yáñez-Mó M, Ramírez MÁ
International journal of molecular sciences 2021 May 26;22(11)
International journal of molecular sciences 2021 May 26;22(11)
miR‑205 suppresses cell migration, invasion and EMT of colon cancer by targeting mouse double minute 4.
Fan Y, Wang K
Molecular medicine reports 2020 Aug;22(2):633-642
Molecular medicine reports 2020 Aug;22(2):633-642
Silence of Stomatin-Like Protein 2 Represses Migration and Invasion Ability of Human Liver Cancer Cells via Inhibiting the Nuclear Factor Kappa B (NF-κB) Pathway.
Zhu W, Li W, Geng Q, Wang X, Sun W, Jiang H, Pu X
Medical science monitor : international medical journal of experimental and clinical research 2018 Oct 25;24:7625-7632
Medical science monitor : international medical journal of experimental and clinical research 2018 Oct 25;24:7625-7632
Forkhead box C2 promotes the invasion ability of human trophoblast cells through Hedgehog (Hh) signaling pathway.
Zhang Y, Zhang Y
Cell biology international 2018 Jul;42(7):859-866
Cell biology international 2018 Jul;42(7):859-866
MicroRNA-27a Inhibits Cell Migration and Invasion of Fibroblast-Like Synoviocytes by Targeting Follistatin-Like Protein 1 in Rheumatoid Arthritis.
Shi DL, Shi GR, Xie J, Du XZ, Yang H
Molecules and cells 2016 Aug 31;39(8):611-8
Molecules and cells 2016 Aug 31;39(8):611-8
In vitro assays of rod and cone opsin activity: retinoid analogs as agonists and inverse agonists.
Kono M, Crouch RK
Methods in molecular biology (Clifton, N.J.) 2010;652:85-94
Methods in molecular biology (Clifton, N.J.) 2010;652:85-94
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MMP2 was performed by loading 25 µg of the indicated whole cell lysates and 10 µL Prestained Protein Ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. MMP2 was detected at ~74 kD using a MMP2 mouse monoclonal antibody (Product # MA1-772) at a concentration of 5 µg/mL in blocking buffer overnight at 4°C on a rocking platform, followed by a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Direct ELISA analysis of MMP2 was performed by coating wells of a 96-well plate with 100 µL per well of recombinant MMP2 protein diluted in carbonate/bicarbonate buffer (Product # 28382) at a concentration of 1 µg/mL overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer (Product # 37538), and incubated with 100 µL per well of a MMP2 mouse monoclonal antibody (Product # MA1-772) at a starting concentration of 1 µg/mL and serially diluting 2-fold to a concentration of 31 ng/mL for 1 hour at 37C, followed by a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:10,000 for 30 minutes at 37c. The plate was washed, then detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 10 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid, and absorbances were read on a spectrophotometer at 450-550 nm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Direct ELISA analysis of MMP2 was performed by coating wells of a 96-well plate with 100 µL per well of recombinant MMP2 protein diluted in carbonate/bicarbonate buffer (Product # 28382) at a concentration of 1 µg/mL overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer (Product # 37538), and incubated with 100 µL per well of a MMP2 mouse monoclonal antibody (Product # MA1-772) at a starting concentration of 1 µg/mL and serially diluting 2-fold to a concentration of 31 ng/mL for 1 hour at 37C, followed by a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:10,000 for 30 minutes at 37c. The plate was washed, then detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 10 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid, and absorbances were read on a spectrophotometer at 450-550 nm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3. Effects of miR-27a on the expression of migration and invasion-related proteins in RA-FLS. RA-FLS were transfected with miR-27a mimic or miR-27a inhibitor. (A) The protein and mRNA expression of MMP2, MMP9, and MMP13 in RA-FLS was detected by western blot and qRT-PCR assay. (B) The protein and mRNA expression of Rac1, Cdc42, and RhoA in RA-FLS was detected by western blot and qRT-PCR assay. * p < 0.05, versus control mimic group. # p < 0.05, versus control inhibitor group.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 BBT secret implantation proteins on EVs, and their secretion pattern, which is altered after the uptake of MSC-EVs or the presence of uterine cytokines. SEC-elution profile of EVs from BBT-9 and BBT-18 for the different experimental conditions (Control, Activin A, Activin A+FLST, pbMSC-EVs, tau-pbMSC-EVs, eMSC-EVs, and tau-eMSC-EVs) were analyzed by Dot Blot using an anti-CD9 specific antibody. The positive EV fractions of each experimental condition were quantified by ImageJ software analysis of the dot blot results ( a ). Bead-assisted flow cytometry analysis of selected implantation proteins expressed in BBT-9- ( b ) and BBT-18-EVs ( c ) after cell stimulation.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. miR-205 mediates the expressions of epithelial-mesenchymal transition-associated factors. (A) Reverse transcription-quantitative PCR was carried out to determine the mRNA levels of E-cadherin, N-cadherin, vimentin, MMP2, and MMP9. (B) Protein expressions of E-cadherin, N-cadherin, vimentin, MMP2, and MMP9 were detected by western blot assay. *P