IC9023P

antibody from R&D Systems

Targeting: MMP2 CLG4, CLG4A, TBE-1

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC9023P

Provider

R&D Systems

Product name

Human MMP-2 PE-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects the pro and active forms of human MMP-2 in Western blots. In dot blots, no cross-reactivity with recombinant human MMP-1, -3, -7, -8, -9, -10, -12, or -13 is observed.

Reactivity

Human

Host

Mouse

Antigen sequence

P08253


Isotype

IgG

Antibody clone number

1A10

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

References

Submitted references

Role of matrix metalloproteinases 2 and 9, toll-like receptor 4 and platelet-leukocyte aggregate formation in sepsis-associated thrombocytopenia.

Larkin CM, Hante NK, Breen EP, Tomaszewski KA, Eisele S, Radomski MW, Ryan TA, Santos-Martinez MJ
PloS one 2018;13(5):e0196478

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MMP9 integrates multiple immunoregulatory pathways that discriminate high suppressive activity of human mesenchymal stem cells.

Lavini-Ramos C, Silva HM, Soares-Schanoski A, Monteiro SM, Ferreira LRP, Pacanaro AP, Gomes S, Batista J, Faé K, Kalil J, Coelho V
Scientific reports 2017 Apr 13;7(1):874

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of MMP-2 in MG-63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human MMP-2 PE-conjugated Monoclonal Antibody (Catalog # IC9023P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.