Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NBP2-59699 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal MMP-9 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detecs ~92kDa and ~82kDa (pro and active forms).
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Streptococcus agalactiae Induces Placental Macrophages To Release Extracellular Traps Loaded with Tissue Remodeling Enzymes via an Oxidative Burst-Dependent Mechanism.
Doster RS, Sutton JA, Rogers LM, Aronoff DM, Gaddy JA
mBio 2018 Nov 20;9(6)
mBio 2018 Nov 20;9(6)
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MMP-9 Antibody (S51-82) [NBP2-59699] - Analysis of Rat Brain showing detection of ~92 kDa and ~82 kDa (pro and active) MMP9 protein using Mouse Anti-MMP9 Monoclonal Antibody, Clone S51-82 . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MMP9 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~92 kDa and ~82 kDa (pro and active).