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- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Flow cytometry [2]
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- Product number
- IC5868A - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Arginase 1/ARG1/liver Arginase Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references STAT6/Arg1 promotes microglia/macrophage efferocytosis and inflammation resolution in stroke mice.
IL-6 mediates ER expansion during hyperpolarization of alternatively activated macrophages.
Pro-inflammatory monocyte profile in patients with major depressive disorder and suicide behaviour and how ketamine induces anti-inflammatory M2 macrophages by NMDAR and mTOR.
ILC2 transfers to apolipoprotein E deficient mice reduce the lipid content of atherosclerotic lesions.
All trans-retinoic acid protects against acute ischemic stroke by modulating neutrophil functions through STAT1 signaling.
CCR2-dependent monocytes/macrophages exacerbate acute brain injury but promote functional recovery after ischemic stroke in mice.
Schwann Cell Transplantation Subdues the Pro-Inflammatory Innate Immune Cell Response after Spinal Cord Injury.
Compensatory upregulation of PD-1, LAG-3, and CTLA-4 limits the efficacy of single-agent checkpoint blockade in metastatic ovarian cancer.
Overexpression of OSM and IL-6 impacts the polarization of pro-fibrotic macrophages and the development of bleomycin-induced lung fibrosis.
Deletion of astroglial CXCL10 delays clinical onset but does not affect progressive axon loss in a murine autoimmune multiple sclerosis model.
Conditional ablation of astroglial CCL2 suppresses CNS accumulation of M1 macrophages and preserves axons in mice with MOG peptide EAE.
Cai W, Dai X, Chen J, Zhao J, Xu M, Zhang L, Yang B, Zhang W, Rocha M, Nakao T, Kofler J, Shi Y, Stetler RA, Hu X, Chen J
JCI insight 2019 Oct 17;4(20)
JCI insight 2019 Oct 17;4(20)
IL-6 mediates ER expansion during hyperpolarization of alternatively activated macrophages.
Ayaub EA, Tandon K, Padwal M, Imani J, Patel H, Dubey A, Mekhael O, Upagupta C, Ayoub A, Dvorkin-Gheva A, Murphy J, Kolb PS, Lhotak S, Dickhout JG, Austin RC, Kolb MRJ, Richards CD, Ask K
Immunology and cell biology 2019 Feb;97(2):203-217
Immunology and cell biology 2019 Feb;97(2):203-217
Pro-inflammatory monocyte profile in patients with major depressive disorder and suicide behaviour and how ketamine induces anti-inflammatory M2 macrophages by NMDAR and mTOR.
Nowak W, Grendas LN, Sanmarco LM, Estecho IG, Arena ÁR, Eberhardt N, Rodante DE, Aoki MP, Daray FM, Carrera Silva EA, Errasti AE
EBioMedicine 2019 Dec;50:290-305
EBioMedicine 2019 Dec;50:290-305
ILC2 transfers to apolipoprotein E deficient mice reduce the lipid content of atherosclerotic lesions.
Mantani PT, Dunér P, Ljungcrantz I, Nilsson J, Björkbacka H, Fredrikson GN
BMC immunology 2019 Dec 10;20(1):47
BMC immunology 2019 Dec 10;20(1):47
All trans-retinoic acid protects against acute ischemic stroke by modulating neutrophil functions through STAT1 signaling.
Cai W, Wang J, Hu M, Chen X, Lu Z, Bellanti JA, Zheng SG
Journal of neuroinflammation 2019 Aug 31;16(1):175
Journal of neuroinflammation 2019 Aug 31;16(1):175
CCR2-dependent monocytes/macrophages exacerbate acute brain injury but promote functional recovery after ischemic stroke in mice.
Fang W, Zhai X, Han D, Xiong X, Wang T, Zeng X, He S, Liu R, Miyata M, Xu B, Zhao H
Theranostics 2018;8(13):3530-3543
Theranostics 2018;8(13):3530-3543
Schwann Cell Transplantation Subdues the Pro-Inflammatory Innate Immune Cell Response after Spinal Cord Injury.
Pearse DD, Bastidas J, Izabel SS, Ghosh M
International journal of molecular sciences 2018 Aug 28;19(9)
International journal of molecular sciences 2018 Aug 28;19(9)
Compensatory upregulation of PD-1, LAG-3, and CTLA-4 limits the efficacy of single-agent checkpoint blockade in metastatic ovarian cancer.
Huang RY, Francois A, McGray AR, Miliotto A, Odunsi K
Oncoimmunology 2017;6(1):e1249561
Oncoimmunology 2017;6(1):e1249561
Overexpression of OSM and IL-6 impacts the polarization of pro-fibrotic macrophages and the development of bleomycin-induced lung fibrosis.
Ayaub EA, Dubey A, Imani J, Botelho F, Kolb MRJ, Richards CD, Ask K
Scientific reports 2017 Oct 16;7(1):13281
Scientific reports 2017 Oct 16;7(1):13281
Deletion of astroglial CXCL10 delays clinical onset but does not affect progressive axon loss in a murine autoimmune multiple sclerosis model.
Mills Ko E, Ma JH, Guo F, Miers L, Lee E, Bannerman P, Burns T, Ko D, Sohn J, Soulika AM, Pleasure D
Journal of neuroinflammation 2014 Jun 12;11:105
Journal of neuroinflammation 2014 Jun 12;11:105
Conditional ablation of astroglial CCL2 suppresses CNS accumulation of M1 macrophages and preserves axons in mice with MOG peptide EAE.
Moreno M, Bannerman P, Ma J, Guo F, Miers L, Soulika AM, Pleasure D
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Jun 11;34(24):8175-85
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Jun 11;34(24):8175-85
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Arginase 1/ARG1 in Hepa 1-6 Mouse Cell Line by Flow Cytometry. Hepa 1-6 mouse hepatoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
