14-9779-80

antibody from Invitrogen Antibodies

Targeting: ARG1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

14-9779-80

Provider

Invitrogen Antibodies

Product name

Arginase 1 Monoclonal Antibody (sl6arg), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: MFNVMVDAK AQSTKLCSME MGQEFAKMWH QYHSKIDELI EETVKEMITL LVAKFVTILE GVLAKLSRYD EGTLFSSFLS FTVKAASKYV DVPKPGMDVA DAYVTFVRHS QDVLRDKVNE EMYIERLFDQ WYNSSMNVIC TWLTDRMDLQ LHIYQLKTLI RMVKKTYRDF RLQGVLDSTL NSKTYETIRN RLTVEEATAS VSEGGGLQGI SMKDSDEEDE EDD (1-222 aa encoded by BC015754)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

sl6arg

Vial size

25 µg

Concentration

0.5 mg/mL

Storage

4° C

References

Submitted references

Deficient Adipogenesis of Scleroderma Patient and Healthy African American Monocytes.

Lee R, Reese C, Carmen-Lopez G, Perry B, Bonner M, Zemskova M, Wilson CL, Helke KL, Silver RM, Hoffman S, Tourkina E
Frontiers in pharmacology 2017;8:174

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Metabolism of L-arginine by myeloid-derived suppressor cells in cancer: mechanisms of T cell suppression and therapeutic perspectives.

Raber P, Ochoa AC, Rodríguez PC
Immunological investigations 2012;41(6-7):614-34

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Arginase-1 is a more sensitive marker of hepatic differentiation than HepPar-1 and glypican-3 in fine-needle aspiration biopsies.

Fujiwara M, Kwok S, Yano H, Pai RK
Cancer cytopathology 2012 Aug 25;120(4):230-7

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Characterization of cytokine-induced myeloid-derived suppressor cells from normal human peripheral blood mononuclear cells.

Lechner MG, Liebertz DJ, Epstein AL
Journal of immunology (Baltimore, Md. : 1950) 2010 Aug 15;185(4):2273-84

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Arginase-1-expressing macrophages suppress Th2 cytokine-driven inflammation and fibrosis.

Pesce JT, Ramalingam TR, Mentink-Kane MM, Wilson MS, El Kasmi KC, Smith AM, Thompson RW, Cheever AW, Murray PJ, Wynn TA
PLoS pathogens 2009 Apr;5(4):e1000371

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Alternative metabolic states in murine macrophages reflected by the nitric oxide synthase/arginase balance: competitive regulation by CD4+ T cells correlates with Th1/Th2 phenotype.

Munder M, Eichmann K, Modolell M
Journal of immunology (Baltimore, Md. : 1950) 1998 Jun 1;160(11):5347-54

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Arginase 1 Monoclonal Antibody (sl6arg), eBioscience™ (Product # 14-9779-82) and a 35kDa band corresponding to Arginase-1 was observed. Whole cell extracts (40 µg lysate) of MOLT-4 (Lane 1) and Hep G2 (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of formalin-fixed paraffin embedded human liver tissue using 5 µg/mL of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (left) or 5 µg/mL of Anti-Human Arginase-1 Purified (right), followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.