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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Western blot [3]
- Immunohistochemistry [7]
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- Product number
- NBP1-87402 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87402, RRID:AB_11036439
- Product name
- Rabbit Polyclonal Occludin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Occludin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The effect of high mobility group box-1 protein on cerebral edema, blood-brain barrier, oxidative stress and apoptosis in an experimental traumatic brain injury model.
Idebenone Protects against Acute Murine Colitis via Antioxidant and Anti-Inflammatory Mechanisms.
Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases.
Electronic cigarette exposure disrupts blood-brain barrier integrity and promotes neuroinflammation.
Hyperglycemia-Driven Neuroinflammation Compromises BBB Leading to Memory Loss in Both Diabetes Mellitus (DM) Type 1 and Type 2 Mouse Models.
Expression of BK channels and Na(+)-K(+) pumps in the apical membrane of lacrimal acinar cells suggests a new molecular mechanism for primary tear-secretion.
Ectopic expression of OX1R in ulcerative colitis mediates anti-inflammatory effect of orexin-A.
Clostridium butyricum exerts a neuroprotective effect in a mouse model of traumatic brain injury via the gut-brain axis.
The Human "Cochlear Battery" - Claudin-11 Barrier and Ion Transport Proteins in the Lateral Wall of the Cochlea.
Malignant ascites determine the transmesothelial invasion of ovarian cancer cells.
Evran S, Calis F, Akkaya E, Baran O, Cevik S, Katar S, Gurevin EG, Hanimoglu H, Hatiboglu MA, Armutak EI, Karatas E, Kocyigit A, Kaynar MY
Brain research bulletin 2020 Jan;154:68-80
Brain research bulletin 2020 Jan;154:68-80
Idebenone Protects against Acute Murine Colitis via Antioxidant and Anti-Inflammatory Mechanisms.
Shastri S, Shinde T, Sohal SS, Gueven N, Eri R
International journal of molecular sciences 2020 Jan 12;21(2)
International journal of molecular sciences 2020 Jan 12;21(2)
Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases.
Shinde T, Perera AP, Vemuri R, Gondalia SV, Beale DJ, Karpe AV, Shastri S, Basheer W, Southam B, Eri R, Stanley R
European journal of nutrition 2020 Dec;59(8):3669-3689
European journal of nutrition 2020 Dec;59(8):3669-3689
Electronic cigarette exposure disrupts blood-brain barrier integrity and promotes neuroinflammation.
Heldt NA, Seliga A, Winfield M, Gajghate S, Reichenbach N, Yu X, Rom S, Tenneti A, May D, Gregory BD, Persidsky Y
Brain, behavior, and immunity 2020 Aug;88:363-380
Brain, behavior, and immunity 2020 Aug;88:363-380
Hyperglycemia-Driven Neuroinflammation Compromises BBB Leading to Memory Loss in Both Diabetes Mellitus (DM) Type 1 and Type 2 Mouse Models.
Rom S, Zuluaga-Ramirez V, Gajghate S, Seliga A, Winfield M, Heldt NA, Kolpakov MA, Bashkirova YV, Sabri AK, Persidsky Y
Molecular neurobiology 2019 Mar;56(3):1883-1896
Molecular neurobiology 2019 Mar;56(3):1883-1896
Expression of BK channels and Na(+)-K(+) pumps in the apical membrane of lacrimal acinar cells suggests a new molecular mechanism for primary tear-secretion.
Almássy J, Diszházi G, Skaliczki M, Márton I, Magyar ZÉ, Nánási PP, Yule DI
The ocular surface 2019 Apr;17(2):272-277
The ocular surface 2019 Apr;17(2):272-277
Ectopic expression of OX1R in ulcerative colitis mediates anti-inflammatory effect of orexin-A.
Messal N, Fernandez N, Dayot S, Gratio V, Nicole P, Prochasson C, Chantret I, LeGuilloux G, Jarry A, Couvelard A, Tréton X, Voisin T, Ogier-Denis E, Couvineau A
Biochimica et biophysica acta. Molecular basis of disease 2018 Nov;1864(11):3618-3628
Biochimica et biophysica acta. Molecular basis of disease 2018 Nov;1864(11):3618-3628
Clostridium butyricum exerts a neuroprotective effect in a mouse model of traumatic brain injury via the gut-brain axis.
Li H, Sun J, Du J, Wang F, Fang R, Yu C, Xiong J, Chen W, Lu Z, Liu J
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society 2018 May;30(5):e13260
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society 2018 May;30(5):e13260
The Human "Cochlear Battery" - Claudin-11 Barrier and Ion Transport Proteins in the Lateral Wall of the Cochlea.
Liu W, Schrott-Fischer A, Glueckert R, Benav H, Rask-Andersen H
Frontiers in molecular neuroscience 2017;10:239
Frontiers in molecular neuroscience 2017;10:239
Malignant ascites determine the transmesothelial invasion of ovarian cancer cells.
Mikuła-Pietrasik J, Uruski P, Szubert S, Szpurek D, Sajdak S, Tykarski A, Książek K
The international journal of biochemistry & cell biology 2017 Nov;92:6-13
The international journal of biochemistry & cell biology 2017 Nov;92:6-13
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Occludin Antibody [NBP1-87402] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Occludin Antibody [NBP1-87402] - Lane 1: untreated vascular endothelial cells Lane 2: vascular endothelial cells + control siRNA. Primary antibody was diluted 1:1000. Image submitted by a verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Occludin Antibody [NBP1-87402] - Analysis in human cell line CACO-2 and human cell line HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human skeletal muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining in human thyroid gland and skeletal muscle tissues using anti-OCLN antibody. Corresponding OCLN RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human thyroid gland shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human breast cancer shows moderate membranous positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human prostate cancer shows moderate membranous positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human stomach shows moderate membranous positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Occludin Antibody [NBP1-87402] - Staining of human prostate shows negative membranous positivity in glandular cells as expected.
