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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-30230 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Occludin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2.
- Concentration
- 0.98 mg/mL
Submitted references EMT alterations in the solute carrier landscape uncover SLC22A10/A15 imposed vulnerabilities in pancreatic cancer.
Low expression of occludin: a predictor of poor prognosis in esophageal squamous cell carcinoma.
Nayak D, Weadick B, Persaud AK, Raj R, Shakya R, Li J, Campbell MJ, Govindarajan R
iScience 2022 May 20;25(5):104193
iScience 2022 May 20;25(5):104193
Low expression of occludin: a predictor of poor prognosis in esophageal squamous cell carcinoma.
Qin Z, Fang D, Fang Y
International journal of clinical and experimental pathology 2017;10(7):7451-7459
International journal of clinical and experimental pathology 2017;10(7):7451-7459
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Occludin using 30 µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Occludin polyclonal antibody (Product # PA5-30230) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Occludin Polyclonal Antibody detects Occludin protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Occludin stained by Occludin Polyclonal Antibody (Product # PA5-30230) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of Occludin was performed in frozen sectioned E13.5 Rat brain tissue using Occludin Polyclonal Antibody (Product # PA5-30230) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using Occludin (Product # PA5-30230) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
