LS-C483078

antibody from LSBio

Targeting: TJP2 DFNA51, X104, ZO-2, ZO2

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C483078

Provider

LSBio

Product name

TJP2 / ZO2 / ZO-2 Antibody (aa740-790) LS-C483078

Antibody type

Polyclonal

Description

Antiserum

Reactivity

Human

Host

Rabbit

Storage

Maintain lyophilized and reconstituted antibodies at -20°C for long term storage and at 2°C to 8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze/thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human large intestine tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human skin tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human skin tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human uterus tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human breast tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human prostate tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human stomach tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human stomach tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human stomach tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human brain tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human appendix tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human large intestine tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human large intestine tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human stomach tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human large intestine tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human skin tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human skin tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human uterus tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human breast tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human prostate tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human stomach tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human stomach tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human stomach tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human brain tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human appendix tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human large intestine tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human large intestine tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human stomach tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human skin tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human skin tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human prostate tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human stomach tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human brain tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human appendix tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rabbit antibody to ZO2 (740-790). IHC on paraffin sections of human stomach tissue using Rabbit antibody to ZO2 (740-790). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.