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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA1-25346 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-SOD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-25346 detects MnSOD from rat, mouse, and human samples. PA1-25346 is expected to cross react with human and mouse due to sequence homology. PA1-25346 has been successfully used in Western blot procedures. The PA1-25346 immunogen is a synthetic peptide corresponding to a portion of human MnSOD encoded within exon 5.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot of SOD2 in whole cell lysate (30µg) from rat vascular smooth muscle cells treated with IL-1 beta (3 ng/ml) for 24 hours (+) or untreated (-) using a SOD2 polyclonal antibody (Product # PA1-25346) at a dilution of 0.4µg/ml.
