Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-65391 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-65391, RRID:AB_960664
- Product name
- Mouse Monoclonal P-Selectin/CD62P Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. NB100-65391 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells. Clone Psel.KO.2.12 is reported to inhibit P-selectin-dependent adhesion between HL60 cells and P-selectin transfected COS cells.
- Reactivity
- Human, Rat, Goat, Sheep
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.12) [NB100-65391] - Figure A. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control. Figure B . FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse anti Human CD62P. All experiments performed on human blood gated on single platelets activated with thrombin, in the presence of 10% Human serum.