Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [2]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BMS108FI - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD54 (ICAM-1) Monoclonal Antibody (RR1/1), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: Recognizes the D1 domain of human CD54.
- Conjugate
- Green dye
- Antibody clone number
- RR1/1
- Concentration
- 10 µL/Test
Submitted references Azelnidipine Inhibits the Differentiation and Activation of THP-1 Macrophages through the L-Type Calcium Channel.
Interleukin-27 enhances TNF-α-mediated activation of human coronary artery endothelial cells.
Identification of plumericin as a potent new inhibitor of the NF-κB pathway with anti-inflammatory activity in vitro and in vivo.
Komoda H, Shiraki A, Oyama JI, Nishikido T, Node K
Journal of atherosclerosis and thrombosis 2018 Aug 1;25(8):690-697
Journal of atherosclerosis and thrombosis 2018 Aug 1;25(8):690-697
Interleukin-27 enhances TNF-α-mediated activation of human coronary artery endothelial cells.
Qiu HN, Liu B, Liu W, Liu S
Molecular and cellular biochemistry 2016 Jan;411(1-2):1-10
Molecular and cellular biochemistry 2016 Jan;411(1-2):1-10
Identification of plumericin as a potent new inhibitor of the NF-κB pathway with anti-inflammatory activity in vitro and in vivo.
Fakhrudin N, Waltenberger B, Cabaravdic M, Atanasov AG, Malainer C, Schachner D, Heiss EH, Liu R, Noha SM, Grzywacz AM, Mihaly-Bison J, Awad EM, Schuster D, Breuss JM, Rollinger JM, Bochkov V, Stuppner H, Dirsch VM
British journal of pharmacology 2014 Apr;171(7):1676-86
British journal of pharmacology 2014 Apr;171(7):1676-86
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout of ICAM-1 (CD54) was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR845355_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD54 was performed by staining Raji CD54 Knock out cells (blue histogram) or Raji Cas9 control cells (pink histogram) with10 µl CD54 (ICAM-1) Monoclonal Antibody (RR1/1), FITC, eBioscience™ (Product # BMS108FI). Lossof signal was observed in the CD54 KOcells stained with CD54 antibody clone RR1/1 but not in the control Cas9cells. Fixable Viability DyeeFluor780 (Product # 65-0865-18) was used for staining and selecting viable cells for analysis.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue and CD54 FITC. As expected based on known relative expression patterns, CD54 clone RR1/1 stains a subset of lymphocytes (pink), monocytes (orange) and granulocytes (blue).
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4. Expressions of (A) ICAM-1 or (B) LOX-1 in THP-1 cells were measured by flow cytometry. * p < 0.05 vs. dimethyl sulfoxide (DMSO, n = 5). (C) Representative western blotting images of MAP kinase. Experiments were repeated three times.
- Conjugate
- Green dye
