Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27429 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- hnRNP A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2.
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of hnRNP 1 using 30 µg of A) 293T (B) A431 (C) HeLa and D) HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a hnRNP 1 polyclonal antibody (Product # PA5-27429) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using hnRNP A1 Polyclonal Antibody (Product # PA5-27429). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A1 Polyclonal Antibody (Product # PA5-27429) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded AGS xenograft, using hnRNP 1 (Product # PA5-27429) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- hnRNP A1 Polyclonal Antibody detects PTBP1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PTBP1 stained by hnRNP A1 Polyclonal Antibody (Product # PA5-27429) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
