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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- AF1699 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Canine CD11b/Integrin alpha M Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CD11b/Integrin alpha M in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse Integrin alpha M and recombinant human Integrin alpha E is observed.
- Reactivity
- Human, Canine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_001139280- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation.
Jerke U, Rolle S, Dittmar G, Bayat B, Santoso S, Sporbert A, Luft F, Kettritz R
The Journal of biological chemistry 2011 Mar 4;286(9):7070-81
The Journal of biological chemistry 2011 Mar 4;286(9):7070-81
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CD11b/Integrin alpha M in Canine PBMCs by Flow Cytometry. Canine peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Canine CD11b/Integrin alpha M Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1699, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
