MHCD1404

antibody from Invitrogen Antibodies

Targeting: CD14

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MHCD1404

Provider

Invitrogen Antibodies

Product name

CD14 Monoclonal Antibody (TuK4), PE

Antibody type

Monoclonal

Antigen

Other

Description

R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Isotype

IgG

Antibody clone number

TuK4

Vial size

500 µL

Storage

4° C, store in dark

References

Submitted references

miR-181a Modulation of ERK-MAPK Signaling Sustains DC-SIGN Expression and Limits Activation of Monocyte-Derived Dendritic Cells.

Lim CX, Lee B, Geiger O, Passegger C, Beitzinger M, Romberger J, Stracke A, Högenauer C, Stift A, Stoiber H, Poidinger M, Zebisch A, Meister G, Williams A, Flavell RA, Henao-Mejia J, Strobl H
Cell reports 2020 Mar 17;30(11):3793-3805.e5

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Physical exercise and human adipose-derived mesenchymal stem cells ameliorate motor disturbances in a male rat model of Parkinson's disease.

Cucarián JD, Berrío JP, Rodrigues C, Zancan M, Wink MR, de Oliveira A
Journal of neuroscience research 2019 Sep;97(9):1095-1109

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Analysis of the safety of mesenchymal stromal cells secretome for glioblastoma treatment.

Onzi GR, Ledur PF, Hainzenreder LD, Bertoni AP, Silva AO, Lenz G, Wink MR
Cytotherapy 2016 Jul;18(7):828-37

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Eradication of Canine Diffuse Large B-Cell Lymphoma in a Murine Xenograft Model with CD47 Blockade and Anti-CD20.

Weiskopf K, Anderson KL, Ito D, Schnorr PJ, Tomiyasu H, Ring AM, Bloink K, Efe J, Rue S, Lowery D, Barkal A, Prohaska S, McKenna KM, Cornax I, O'Brien TD, O'Sullivan MG, Weissman IL, Modiano JF
Cancer immunology research 2016 Dec;4(12):1072-1087

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Antibacterial responses by peritoneal macrophages are enhanced following vitamin D supplementation.

Bacchetta J, Chun RF, Gales B, Zaritsky JJ, Leroy S, Wesseling-Perry K, Boregaard N, Rastogi A, Salusky IB, Hewison M
PloS one 2014;9(12):e116530

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Coexpression of CD14 and CD326 discriminate hepatic precursors in the human fetal liver.

Fomin ME, Tai LK, Bárcena A, Muench MO
Stem cells and development 2011 Jul;20(7):1247-57

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The endogenous danger signals HSP70 and MICA cooperate in the activation of cytotoxic effector functions of NK cells.

Elsner L, Flügge PF, Lozano J, Muppala V, Eiz-Vesper B, Demiroglu SY, Malzahn D, Herrmann T, Brunner E, Bickeböller H, Multhoff G, Walter L, Dressel R
Journal of cellular and molecular medicine 2010 Apr;14(4):992-1002

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Preclinical evaluation of carcinoembryonic cell adhesion molecule (CEACAM) 6 as potential therapy target for pancreatic adenocarcinoma.

Strickland LA, Ross J, Williams S, Ross S, Romero M, Spencer S, Erickson R, Sutcliffe J, Verbeke C, Polakis P, van Bruggen N, Koeppen H
The Journal of pathology 2009 Jul;218(3):380-90

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Evaluation of leukocyte stabilisation in TransFix-treated blood samples by flow cytometry and transmission electron microscopy.

Canonico B, Zamai L, Burattini S, Granger V, Mannello F, Gobbi P, Felici C, Falcieri E, Reilly JT, Barnett D, Papa S
Journal of immunological methods 2004 Dec;295(1-2):67-78

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human peripheral blood monocytes stained using R-PE of anti-human CD14 monoclonal antibody (clone Tuk4). The negative control profiles represent unstained cells.

Conjugate

Yellow dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Yellow dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Yellow dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Yellow dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Morphological features and characterization of ADSCs. Our mesenchymal stromal cells had a spindle-shaped, fibroblast-like morphology, and demonstrated plastic adherent properties in culture. Cells underwent differentiation in (a) chondrocytes stained by Alcian blue; (c) Adipocytes showing some lipid vesicles stained by Oil red; and (e) osteoblasts, showing calcium phosphate stained with Alizarin red, after 3 weeks of induction. The negative controls, which received only the standard culture medium at the same time as those induced are showing for comparison (b,d,f). (g) Histograms of flow cytometry showing that these cells were homogeneously positive for mesenchymal markers: CD44, and CD105; but negative for hematopoietic markers: CD14, CD34, and CD45. Microphotograph scale = 50 mum [Color figure can be viewed at wileyonlinelibrary.com ]

Conjugate

Yellow dye