Antibody data
- Antibody Data
- Antigen structure
- References [70]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [28]
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Validation data
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- Product number
- 17-0149-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD14 Monoclonal Antibody (61D3), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.
- Antibody clone number
- 61D3
- Concentration
- 5 µL/Test
Submitted references Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus.
A peptide derived from chaperonin 60.1, IRL201104, inhibits LPS-induced acute lung inflammation.
Role of the thymus in spontaneous development of a multi-organ autoimmune disease in human immune system mice.
Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection.
Immunoresponsive Tissue-Engineered Oral Mucosal Equivalents Containing Macrophages.
Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity.
The long non-coding RNA Cancer Susceptibility 15 (CASC15) is induced by isocitrate dehydrogenase (IDH) mutations and maintains an immature phenotype in adult acute myeloid leukemia.
Platelets Fuel the Inflammasome Activation of Innate Immune Cells.
Engineering monocyte/macrophage-specific glucocerebrosidase expression in human hematopoietic stem cells using genome editing.
Targeting FTO Suppresses Cancer Stem Cell Maintenance and Immune Evasion.
Optimizing the Method of Cell Separation from Bile of Patients with Cholangiocarcinoma for Flow Cytometry.
Differential expression and predictive value of monocyte scavenger receptor CD163 in populations with different tuberculosis infection statuses.
Effects of leukemia inhibitory factor receptor on the adipogenic differentiation of human bone marrow mesenchymal stem cells.
Human-like NSG mouse glycoproteins sialylation pattern changes the phenotype of human lymphocytes and sensitivity to HIV-1 infection.
The impact of the rs8005161 polymorphism on G protein-coupled receptor GPR65 (TDAG8) pH-associated activation in intestinal inflammation.
Small-Molecule Targeting of Oncogenic FTO Demethylase in Acute Myeloid Leukemia.
Characterizing the Role of Monocytes in T Cell Cancer Immunotherapy Using a 3D Microfluidic Model.
Toll-Interleukin 1 Receptor Domain-Containing Adaptor Protein 180L Single-Nucleotide Polymorphism Is Associated With Susceptibility to Recurrent Pneumococcal Lower Respiratory Tract Infections in Children.
In Vitro Expansion and Characterization of Mesenchymal Stromal Cells from Peritoneal Dialysis Effluent in a Human Protein Medium.
Butyrate upregulates the TLR4 expression and the phosphorylation of MAPKs and NK-κB in colon cancer cell in vitro.
Genetic and Epigenetic Perturbations by DNMT3A-R882 Mutants Impaired Apoptosis through Augmentation of PRDX2 in Myeloid Leukemia Cells.
Cellular metabolism constrains innate immune responses in early human ontogeny.
Efficient immunoaffinity chromatography of lymphocytes directly from whole blood.
AURKA Suppresses Leukemic THP-1 Cell Differentiation through Inhibition of the KDM6B Pathway.
Monocyte NOTCH2 expression predicts IFN-β immunogenicity in multiple sclerosis patients.
Challenging PD-L1 expressing cytotoxic T cells as a predictor for response to immunotherapy in melanoma.
Hepatocyte-derived exosomes promote T follicular regulatory cell expansion during hepatitis C virus infection.
Cellular stressors contribute to the expansion of hematopoietic clones of varying leukemic potential.
Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.
The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence.
Increased Plasmodium falciparum Parasitemia in Non-splenectomized Saimiri sciureus Monkeys Treated with Clodronate Liposomes.
KSRP specifies monocytic and granulocytic differentiation through regulating miR-129 biogenesis and RUNX1 expression.
Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.
A therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens.
TTI-621 (SIRPαFc): A CD47-Blocking Innate Immune Checkpoint Inhibitor with Broad Antitumor Activity and Minimal Erythrocyte Binding.
LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells.
Quantitative Measurements of HIV-1 and Dextran Capture by Human Monocyte-derived Dendritic Cells (MDDCs).
TNFα promotes CAR-dependent migration of leukocytes across epithelial monolayers.
Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.
Transendothelial migration of human umbilical mesenchymal stem cells across uterine endothelial monolayers: Junctional dynamics and putative mechanisms.
Thrombomodulin regulates monocye differentiation via PKCδ and ERK1/2 pathway in vitro and in atherosclerotic artery.
Investigating the causes for decreased levels of glutathione in individuals with type II diabetes.
Liposomal Glutathione Supplementation Restores TH1 Cytokine Response to Mycobacterium tuberculosis Infection in HIV-Infected Individuals.
Interferon-γ enhances both the anti-bacterial and the pro-inflammatory response of human mast cells to Staphylococcus aureus.
DNA-Containing Immunocomplexes Promote Inflammasome Assembly and Release of Pyrogenic Cytokines by CD14+ CD16+ CD64high CD32low Inflammatory Monocytes from Malaria Patients.
A novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignancies.
Characterization of the in vivo immune network of IDO, tryptophan metabolism, PD-L1, and CTLA-4 in circulating immune cells in melanoma.
Peanut oral immunotherapy transiently expands circulating Ara h 2-specific B cells with a homologous repertoire in unrelated subjects.
Control of signaling-mediated clearance of apoptotic cells by the tumor suppressor p53.
Clinical significance of plasmacytoid dendritic cells and myeloid-derived suppressor cells in melanoma.
Pioglitazone regulates myelin phagocytosis and multiple sclerosis monocytes.
Retinoid X receptor activation reverses age-related deficiencies in myelin debris phagocytosis and remyelination.
Biologically active polymers from spontaneous carotenoid oxidation: a new frontier in carotenoid activity.
The CD14+CD16+ inflammatory monocyte subset displays increased mitochondrial activity and effector function during acute Plasmodium vivax malaria.
Secondary lymphoid organ homing phenotype of human myeloid dendritic cells disrupted by an intracellular oral pathogen.
Human mesenchymal stem cells possess different biological characteristics but do not change their therapeutic potential when cultured in serum free medium.
Soluble tumor necrosis factor related apoptosis inducing ligand level as a predictor of severity of sepsis and the risk of mortality in septic patients.
MicroRNAs transfer from human macrophages to hepato-carcinoma cells and inhibit proliferation.
Noncanonical dendritic cell differentiation and survival driven by a bacteremic pathogen.
Tumor necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse signaling.
Neutrophil paralysis in Plasmodium vivax malaria.
Negative regulation of JAK2 by H3K9 methyltransferase G9a in leukemia.
Induction of autophagy is essential for monocyte-macrophage differentiation.
Actin polymerization stabilizes α4β1 integrin anchors that mediate monocyte adhesion.
The cell surface proteome of human mesenchymal stromal cells.
Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients.
Ro60-associated single-stranded RNA links inflammation with fetal cardiac fibrosis via ligation of TLRs: a novel pathway to autoimmune-associated heart block.
C5a enhances dysregulated inflammatory and angiogenic responses to malaria in vitro: potential implications for placental malaria.
B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity.
Modulation of dendritic cell maturation and function by the Tax protein of human T cell leukemia virus type 1.
Satria RD, Huang TW, Jhan MK, Shen TJ, Tseng PC, Wang YT, Yang ZY, Hsing CH, Lin CF
Journal of immunology research 2021;2021:6654617
Journal of immunology research 2021;2021:6654617
A peptide derived from chaperonin 60.1, IRL201104, inhibits LPS-induced acute lung inflammation.
Man F, Nadkarni S, Kanabar V, E-Lacerda RR, Gomes Ferreira S, Federici Canova D, Perretti M, Page CP, Riffo-Vasquez Y
American journal of physiology. Lung cellular and molecular physiology 2021 Nov 1;321(5):L803-L813
American journal of physiology. Lung cellular and molecular physiology 2021 Nov 1;321(5):L803-L813
Role of the thymus in spontaneous development of a multi-organ autoimmune disease in human immune system mice.
Khosravi-Maharlooei M, Li H, Hoelzl M, Zhao G, Ruiz A, Misra A, Li Y, Teteloshvili N, Nauman G, Danzl N, Ding X, Pinker EY, Obradovic A, Yang YG, Iuga A, Creusot RJ, Winchester R, Sykes M
Journal of autoimmunity 2021 May;119:102612
Journal of autoimmunity 2021 May;119:102612
Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection.
Oliva Chávez AS, Wang X, Marnin L, Archer NK, Hammond HL, Carroll EEM, Shaw DK, Tully BG, Buskirk AD, Ford SL, Butler LR, Shahi P, Morozova K, Clement CC, Lawres L, Neal AJO, Mamoun CB, Mason KL, Hobbs BE, Scoles GA, Barry EM, Sonenshine DE, Pal U, Valenzuela JG, Sztein MB, Pasetti MF, Levin ML, Kotsyfakis M, Jay SM, Huntley JF, Miller LS, Santambrogio L, Pedra JHF
Nature communications 2021 Jun 17;12(1):3696
Nature communications 2021 Jun 17;12(1):3696
Immunoresponsive Tissue-Engineered Oral Mucosal Equivalents Containing Macrophages.
Ollington B, Colley HE, Murdoch C
Tissue engineering. Part C, Methods 2021 Aug;27(8):462-471
Tissue engineering. Part C, Methods 2021 Aug;27(8):462-471
Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity.
Oggero S, de Gaetano M, Marcone S, Fitzsimons S, Pinto AL, Ikramova D, Barry M, Burke D, Montero-Melendez T, Cooper D, Burgoyne T, Belton O, Norling LV, Brennan EP, Godson C, Perretti M
Journal of extracellular vesicles 2021 Apr;10(6):12084
Journal of extracellular vesicles 2021 Apr;10(6):12084
The long non-coding RNA Cancer Susceptibility 15 (CASC15) is induced by isocitrate dehydrogenase (IDH) mutations and maintains an immature phenotype in adult acute myeloid leukemia.
Grasedieck S, Ruess C, Krowiorz K, Lux S, Pochert N, Schwarzer A, Klusmann JH, Jongen-Lavrencic M, Herold T, Bullinger L, Pollack JR, Rouhi A, Kuchenbauer F
Haematologica 2020 Sep 1;105(9):e448-453
Haematologica 2020 Sep 1;105(9):e448-453
Platelets Fuel the Inflammasome Activation of Innate Immune Cells.
Rolfes V, Ribeiro LS, Hawwari I, Böttcher L, Rosero N, Maasewerd S, Santos MLS, Próchnicki T, Silva CMS, Wanderley CWS, Rothe M, Schmidt SV, Stunden HJ, Bertheloot D, Rivas MN, Fontes CJ, Carvalho LH, Cunha FQ, Latz E, Arditi M, Franklin BS
Cell reports 2020 May 12;31(6):107615
Cell reports 2020 May 12;31(6):107615
Engineering monocyte/macrophage-specific glucocerebrosidase expression in human hematopoietic stem cells using genome editing.
Scharenberg SG, Poletto E, Lucot KL, Colella P, Sheikali A, Montine TJ, Porteus MH, Gomez-Ospina N
Nature communications 2020 Jul 3;11(1):3327
Nature communications 2020 Jul 3;11(1):3327
Targeting FTO Suppresses Cancer Stem Cell Maintenance and Immune Evasion.
Su R, Dong L, Li Y, Gao M, Han L, Wunderlich M, Deng X, Li H, Huang Y, Gao L, Li C, Zhao Z, Robinson S, Tan B, Qing Y, Qin X, Prince E, Xie J, Qin H, Li W, Shen C, Sun J, Kulkarni P, Weng H, Huang H, Chen Z, Zhang B, Wu X, Olsen MJ, Müschen M, Marcucci G, Salgia R, Li L, Fathi AT, Li Z, Mulloy JC, Wei M, Horne D, Chen J
Cancer cell 2020 Jul 13;38(1):79-96.e11
Cancer cell 2020 Jul 13;38(1):79-96.e11
Optimizing the Method of Cell Separation from Bile of Patients with Cholangiocarcinoma for Flow Cytometry.
Xia Y, Gao Y, Wang B, Zhang H, Zhang Q
Gastroenterology research and practice 2019;2019:5436961
Gastroenterology research and practice 2019;2019:5436961
Differential expression and predictive value of monocyte scavenger receptor CD163 in populations with different tuberculosis infection statuses.
Liu Q, Ou Q, Chen H, Gao Y, Liu Y, Xu Y, Ruan Q, Zhang W, Shao L
BMC infectious diseases 2019 Nov 28;19(1):1006
BMC infectious diseases 2019 Nov 28;19(1):1006
Effects of leukemia inhibitory factor receptor on the adipogenic differentiation of human bone marrow mesenchymal stem cells.
Wang T, Yan R, Xu X, Yu H, Wu J, Yang Y, Li W
Molecular medicine reports 2019 Jun;19(6):4719-4726
Molecular medicine reports 2019 Jun;19(6):4719-4726
Human-like NSG mouse glycoproteins sialylation pattern changes the phenotype of human lymphocytes and sensitivity to HIV-1 infection.
Dagur RS, Branch-Woods A, Mathews S, Joshi PS, Quadros RM, Harms DW, Cheng Y, Miles SM, Pirruccello SJ, Gurumurthy CB, Gorantla S, Poluektova LY
BMC immunology 2019 Jan 7;20(1):2
BMC immunology 2019 Jan 7;20(1):2
The impact of the rs8005161 polymorphism on G protein-coupled receptor GPR65 (TDAG8) pH-associated activation in intestinal inflammation.
Tcymbarevich IV, Eloranta JJ, Rossel JB, Obialo N, Spalinger M, Cosin-Roger J, Lang S, Kullak-Ublick GA, Wagner CA, Scharl M, Seuwen K, Ruiz PA, Rogler G, de Vallière C, Misselwitz B, Swiss IBD Cohort Study Group
BMC gastroenterology 2019 Jan 7;19(1):2
BMC gastroenterology 2019 Jan 7;19(1):2
Small-Molecule Targeting of Oncogenic FTO Demethylase in Acute Myeloid Leukemia.
Huang Y, Su R, Sheng Y, Dong L, Dong Z, Xu H, Ni T, Zhang ZS, Zhang T, Li C, Han L, Zhu Z, Lian F, Wei J, Deng Q, Wang Y, Wunderlich M, Gao Z, Pan G, Zhong D, Zhou H, Zhang N, Gan J, Jiang H, Mulloy JC, Qian Z, Chen J, Yang CG
Cancer cell 2019 Apr 15;35(4):677-691.e10
Cancer cell 2019 Apr 15;35(4):677-691.e10
Characterizing the Role of Monocytes in T Cell Cancer Immunotherapy Using a 3D Microfluidic Model.
Lee SWL, Adriani G, Ceccarello E, Pavesi A, Tan AT, Bertoletti A, Kamm RD, Wong SC
Frontiers in immunology 2018;9:416
Frontiers in immunology 2018;9:416
Toll-Interleukin 1 Receptor Domain-Containing Adaptor Protein 180L Single-Nucleotide Polymorphism Is Associated With Susceptibility to Recurrent Pneumococcal Lower Respiratory Tract Infections in Children.
Siebert JN, Hamann L, Verolet CM, Gameiro C, Grillet S, Siegrist CA, Posfay-Barbe KM
Frontiers in immunology 2018;9:1780
Frontiers in immunology 2018;9:1780
In Vitro Expansion and Characterization of Mesenchymal Stromal Cells from Peritoneal Dialysis Effluent in a Human Protein Medium.
Han B, Zhou L, Guan Q, da Roza G, Wang H, Du C
Stem cells international 2018;2018:5868745
Stem cells international 2018;2018:5868745
Butyrate upregulates the TLR4 expression and the phosphorylation of MAPKs and NK-κB in colon cancer cell in vitro.
Xiao T, Wu S, Yan C, Zhao C, Jin H, Yan N, Xu J, Wu Y, Li C, Shao Q, Xia S
Oncology letters 2018 Oct;16(4):4439-4447
Oncology letters 2018 Oct;16(4):4439-4447
Genetic and Epigenetic Perturbations by DNMT3A-R882 Mutants Impaired Apoptosis through Augmentation of PRDX2 in Myeloid Leukemia Cells.
Bera R, Chiu MC, Huang YJ, Liang DC, Lee YS, Shih LY
Neoplasia (New York, N.Y.) 2018 Nov;20(11):1106-1120
Neoplasia (New York, N.Y.) 2018 Nov;20(11):1106-1120
Cellular metabolism constrains innate immune responses in early human ontogeny.
Kan B, Michalski C, Fu H, Au HHT, Lee K, Marchant EA, Cheng MF, Anderson-Baucum E, Aharoni-Simon M, Tilley P, Mirmira RG, Ross CJ, Luciani DS, Jan E, Lavoie PM
Nature communications 2018 Nov 16;9(1):4822
Nature communications 2018 Nov 16;9(1):4822
Efficient immunoaffinity chromatography of lymphocytes directly from whole blood.
Mohr F, Przibilla S, Leonhardt F, Stemberger C, Dreher S, Müller TR, Fräßle SP, Schmidt GP, Kiene ML, Stadler H, Busch DH
Scientific reports 2018 Nov 13;8(1):16731
Scientific reports 2018 Nov 13;8(1):16731
AURKA Suppresses Leukemic THP-1 Cell Differentiation through Inhibition of the KDM6B Pathway.
Park JW, Cho H, Oh H, Kim JY, Seo SB
Molecules and cells 2018 May 31;41(5):444-453
Molecules and cells 2018 May 31;41(5):444-453
Monocyte NOTCH2 expression predicts IFN-β immunogenicity in multiple sclerosis patients.
Adriani M, Nytrova P, Mbogning C, Hässler S, Medek K, Jensen PEH, Creeke P, Warnke C, Ingenhoven K, Hemmer B, Sievers C, Lindberg Gasser RL, Fissolo N, Deisenhammer F, Bocskei Z, Mikol V, Fogdell-Hahn A, Kubala Havrdova E, Broët P, Dönnes P, Mauri C, Jury EC, ABIRISK Consortium
JCI insight 2018 Jun 7;3(11)
JCI insight 2018 Jun 7;3(11)
Challenging PD-L1 expressing cytotoxic T cells as a predictor for response to immunotherapy in melanoma.
Brochez L, Meireson A, Chevolet I, Sundahl N, Ost P, Kruse V
Nature communications 2018 Jul 26;9(1):2921
Nature communications 2018 Jul 26;9(1):2921
Hepatocyte-derived exosomes promote T follicular regulatory cell expansion during hepatitis C virus infection.
Cobb DA, Kim OK, Golden-Mason L, Rosen HR, Hahn YS
Hepatology (Baltimore, Md.) 2018 Jan;67(1):71-85
Hepatology (Baltimore, Md.) 2018 Jan;67(1):71-85
Cellular stressors contribute to the expansion of hematopoietic clones of varying leukemic potential.
Wong TN, Miller CA, Jotte MRM, Bagegni N, Baty JD, Schmidt AP, Cashen AF, Duncavage EJ, Helton NM, Fiala M, Fulton RS, Heath SE, Janke M, Luber K, Westervelt P, Vij R, DiPersio JF, Welch JS, Graubert TA, Walter MJ, Ley TJ, Link DC
Nature communications 2018 Jan 31;9(1):455
Nature communications 2018 Jan 31;9(1):455
Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.
Ventura Ferreira MS, Bienert M, Müller K, Rath B, Goecke T, Opländer C, Braunschweig T, Mela P, Brümmendorf TH, Beier F, Neuss S
Stem cell research & therapy 2018 Feb 5;9(1):28
Stem cell research & therapy 2018 Feb 5;9(1):28
The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence.
Ong SM, Hadadi E, Dang TM, Yeap WH, Tan CT, Ng TP, Larbi A, Wong SC
Cell death & disease 2018 Feb 15;9(3):266
Cell death & disease 2018 Feb 15;9(3):266
Increased Plasmodium falciparum Parasitemia in Non-splenectomized Saimiri sciureus Monkeys Treated with Clodronate Liposomes.
Cunha JA, Carvalho LJM, Bianco-Junior C, Andrade MCR, Pratt-Riccio LR, Riccio EKP, Pelajo-Machado M, da Silva IJ, Druilhe P, Daniel-Ribeiro CT
Frontiers in cellular and infection microbiology 2017;7:408
Frontiers in cellular and infection microbiology 2017;7:408
KSRP specifies monocytic and granulocytic differentiation through regulating miR-129 biogenesis and RUNX1 expression.
Zhao H, Wang X, Yi P, Si Y, Tan P, He J, Yu S, Ren Y, Ma Y, Zhang J, Wang D, Wang F, Yu J
Nature communications 2017 Nov 10;8(1):1428
Nature communications 2017 Nov 10;8(1):1428
Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.
Li J, Mao Q, He J, She H, Zhang Z, Yin C
Stem cell research & therapy 2017 Mar 9;8(1):55
Stem cell research & therapy 2017 Mar 9;8(1):55
A therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens.
Chang AY, Dao T, Gejman RS, Jarvis CA, Scott A, Dubrovsky L, Mathias MD, Korontsvit T, Zakhaleva V, Curcio M, Hendrickson RC, Liu C, Scheinberg DA
The Journal of clinical investigation 2017 Jun 30;127(7):2705-2718
The Journal of clinical investigation 2017 Jun 30;127(7):2705-2718
TTI-621 (SIRPαFc): A CD47-Blocking Innate Immune Checkpoint Inhibitor with Broad Antitumor Activity and Minimal Erythrocyte Binding.
Petrova PS, Viller NN, Wong M, Pang X, Lin GH, Dodge K, Chai V, Chen H, Lee V, House V, Vigo NT, Jin D, Mutukura T, Charbonneau M, Truong T, Viau S, Johnson LD, Linderoth E, Sievers EL, Maleki Vareki S, Figueredo R, Pampillo M, Koropatnick J, Trudel S, Mbong N, Jin L, Wang JC, Uger RA
Clinical cancer research : an official journal of the American Association for Cancer Research 2017 Feb 15;23(4):1068-1079
Clinical cancer research : an official journal of the American Association for Cancer Research 2017 Feb 15;23(4):1068-1079
LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells.
Lu XJ, Chen Q, Rong YJ, Yang GJ, Li CH, Xu NY, Yu CH, Wang HY, Zhang S, Shi YH, Chen J
Nature communications 2016 Sep 6;7:12719
Nature communications 2016 Sep 6;7:12719
Quantitative Measurements of HIV-1 and Dextran Capture by Human Monocyte-derived Dendritic Cells (MDDCs).
Ménager MM, Littman DR
Bio-protocol 2016 Nov 20;6(22)
Bio-protocol 2016 Nov 20;6(22)
TNFα promotes CAR-dependent migration of leukocytes across epithelial monolayers.
Morton PE, Hicks A, Ortiz-Zapater E, Raghavan S, Pike R, Noble A, Woodfin A, Jenkins G, Rayner E, Santis G, Parsons M
Scientific reports 2016 May 19;6:26321
Scientific reports 2016 May 19;6:26321
Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.
Sakthivel P, Grunewald J, Eklund A, Bruder D, Wahlström J
Clinical and experimental immunology 2016 Feb;183(2):294-306
Clinical and experimental immunology 2016 Feb;183(2):294-306
Transendothelial migration of human umbilical mesenchymal stem cells across uterine endothelial monolayers: Junctional dynamics and putative mechanisms.
Ebrahim NA, Leach L
Placenta 2016 Dec;48:87-98
Placenta 2016 Dec;48:87-98
Thrombomodulin regulates monocye differentiation via PKCδ and ERK1/2 pathway in vitro and in atherosclerotic artery.
Tsai CS, Lin YW, Huang CY, Shih CM, Tsai YT, Tsao NW, Lin CS, Shih CC, Jeng H, Lin FY
Scientific reports 2016 Dec 2;6:38421
Scientific reports 2016 Dec 2;6:38421
Investigating the causes for decreased levels of glutathione in individuals with type II diabetes.
Lagman M, Ly J, Saing T, Kaur Singh M, Vera Tudela E, Morris D, Chi PT, Ochoa C, Sathananthan A, Venketaraman V
PloS one 2015;10(3):e0118436
PloS one 2015;10(3):e0118436
Liposomal Glutathione Supplementation Restores TH1 Cytokine Response to Mycobacterium tuberculosis Infection in HIV-Infected Individuals.
Ly J, Lagman M, Saing T, Singh MK, Tudela EV, Morris D, Anderson J, Daliva J, Ochoa C, Patel N, Pearce D, Venketaraman V
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2015 Nov;35(11):875-87
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2015 Nov;35(11):875-87
Interferon-γ enhances both the anti-bacterial and the pro-inflammatory response of human mast cells to Staphylococcus aureus.
Swindle EJ, Brown JM, Rådinger M, DeLeo FR, Metcalfe DD
Immunology 2015 Nov;146(3):470-85
Immunology 2015 Nov;146(3):470-85
DNA-Containing Immunocomplexes Promote Inflammasome Assembly and Release of Pyrogenic Cytokines by CD14+ CD16+ CD64high CD32low Inflammatory Monocytes from Malaria Patients.
Hirako IC, Gallego-Marin C, Ataide MA, Andrade WA, Gravina H, Rocha BC, de Oliveira RB, Pereira DB, Vinetz J, Diamond B, Ram S, Golenbock DT, Gazzinelli RT
mBio 2015 Nov 17;6(6):e01605-15
mBio 2015 Nov 17;6(6):e01605-15
A novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignancies.
Kim SY, Theunissen JW, Balibalos J, Liao-Chan S, Babcock MC, Wong T, Cairns B, Gonzalez D, van der Horst EH, Perez M, Levashova Z, Chinn L, D'Alessio JA, Flory M, Bermudez A, Jackson DY, Ha E, Monteon J, Bruhns MF, Chen G, Migone TS
Blood cancer journal 2015 May 29;5(5):e316
Blood cancer journal 2015 May 29;5(5):e316
Characterization of the in vivo immune network of IDO, tryptophan metabolism, PD-L1, and CTLA-4 in circulating immune cells in melanoma.
Chevolet I, Speeckaert R, Schreuer M, Neyns B, Krysko O, Bachert C, Hennart B, Allorge D, van Geel N, Van Gele M, Brochez L
Oncoimmunology 2015 Mar;4(3):e982382
Oncoimmunology 2015 Mar;4(3):e982382
Peanut oral immunotherapy transiently expands circulating Ara h 2-specific B cells with a homologous repertoire in unrelated subjects.
Patil SU, Ogunniyi AO, Calatroni A, Tadigotla VR, Ruiter B, Ma A, Moon J, Love JC, Shreffler WG
The Journal of allergy and clinical immunology 2015 Jul;136(1):125-134.e12
The Journal of allergy and clinical immunology 2015 Jul;136(1):125-134.e12
Control of signaling-mediated clearance of apoptotic cells by the tumor suppressor p53.
Yoon KW, Byun S, Kwon E, Hwang SY, Chu K, Hiraki M, Jo SH, Weins A, Hakroush S, Cebulla A, Sykes DB, Greka A, Mundel P, Fisher DE, Mandinova A, Lee SW
Science (New York, N.Y.) 2015 Jul 31;349(6247):1261669
Science (New York, N.Y.) 2015 Jul 31;349(6247):1261669
Clinical significance of plasmacytoid dendritic cells and myeloid-derived suppressor cells in melanoma.
Chevolet I, Speeckaert R, Schreuer M, Neyns B, Krysko O, Bachert C, Van Gele M, van Geel N, Brochez L
Journal of translational medicine 2015 Jan 16;13:9
Journal of translational medicine 2015 Jan 16;13:9
Pioglitazone regulates myelin phagocytosis and multiple sclerosis monocytes.
Natrajan MS, Komori M, Kosa P, Johnson KR, Wu T, Franklin RJ, Bielekova B
Annals of clinical and translational neurology 2015 Dec;2(12):1071-84
Annals of clinical and translational neurology 2015 Dec;2(12):1071-84
Retinoid X receptor activation reverses age-related deficiencies in myelin debris phagocytosis and remyelination.
Natrajan MS, de la Fuente AG, Crawford AH, Linehan E, Nuñez V, Johnson KR, Wu T, Fitzgerald DC, Ricote M, Bielekova B, Franklin RJ
Brain : a journal of neurology 2015 Dec;138(Pt 12):3581-97
Brain : a journal of neurology 2015 Dec;138(Pt 12):3581-97
Biologically active polymers from spontaneous carotenoid oxidation: a new frontier in carotenoid activity.
Johnston JB, Nickerson JG, Daroszewski J, Mogg TJ, Burton GW
PloS one 2014;9(10):e111346
PloS one 2014;9(10):e111346
The CD14+CD16+ inflammatory monocyte subset displays increased mitochondrial activity and effector function during acute Plasmodium vivax malaria.
Antonelli LR, Leoratti FM, Costa PA, Rocha BC, Diniz SQ, Tada MS, Pereira DB, Teixeira-Carvalho A, Golenbock DT, Gonçalves R, Gazzinelli RT
PLoS pathogens 2014 Sep;10(9):e1004393
PLoS pathogens 2014 Sep;10(9):e1004393
Secondary lymphoid organ homing phenotype of human myeloid dendritic cells disrupted by an intracellular oral pathogen.
Miles B, Zakhary I, El-Awady A, Scisci E, Carrion J, O'Neill JC, Rawlings A, Stern JK, Susin C, Cutler CW
Infection and immunity 2014 Jan;82(1):101-11
Infection and immunity 2014 Jan;82(1):101-11
Human mesenchymal stem cells possess different biological characteristics but do not change their therapeutic potential when cultured in serum free medium.
Wang Y, Wu H, Yang Z, Chi Y, Meng L, Mao A, Yan S, Hu S, Zhang J, Zhang Y, Yu W, Ma Y, Li T, Cheng Y, Wang Y, Wang S, Liu J, Han J, Li C, Liu L, Xu J, Han ZB, Han ZC
Stem cell research & therapy 2014 Dec 4;5(6):132
Stem cell research & therapy 2014 Dec 4;5(6):132
Soluble tumor necrosis factor related apoptosis inducing ligand level as a predictor of severity of sepsis and the risk of mortality in septic patients.
Tian Y, Tao T, Zhu J, Zou Y, Wang J, Li J, Bo L, Deng X
PloS one 2013;8(12):e82204
PloS one 2013;8(12):e82204
MicroRNAs transfer from human macrophages to hepato-carcinoma cells and inhibit proliferation.
Aucher A, Rudnicka D, Davis DM
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 15;191(12):6250-60
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 15;191(12):6250-60
Noncanonical dendritic cell differentiation and survival driven by a bacteremic pathogen.
Miles B, Scisci E, Carrion J, Sabino GJ, Genco CA, Cutler CW
Journal of leukocyte biology 2013 Aug;94(2):281-9
Journal of leukocyte biology 2013 Aug;94(2):281-9
Tumor necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse signaling.
Moh MC, Lorenzini PA, Gullo C, Schwarz H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013 Aug;27(8):2957-66
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013 Aug;27(8):2957-66
Neutrophil paralysis in Plasmodium vivax malaria.
Leoratti FM, Trevelin SC, Cunha FQ, Rocha BC, Costa PA, Gravina HD, Tada MS, Pereira DB, Golenbock DT, Antonelli LR, Gazzinelli RT
PLoS neglected tropical diseases 2012;6(6):e1710
PLoS neglected tropical diseases 2012;6(6):e1710
Negative regulation of JAK2 by H3K9 methyltransferase G9a in leukemia.
Son HJ, Kim JY, Hahn Y, Seo SB
Molecular and cellular biology 2012 Sep;32(18):3681-94
Molecular and cellular biology 2012 Sep;32(18):3681-94
Induction of autophagy is essential for monocyte-macrophage differentiation.
Zhang Y, Morgan MJ, Chen K, Choksi S, Liu ZG
Blood 2012 Mar 22;119(12):2895-905
Blood 2012 Mar 22;119(12):2895-905
Actin polymerization stabilizes α4β1 integrin anchors that mediate monocyte adhesion.
Rullo J, Becker H, Hyduk SJ, Wong JC, Digby G, Arora PD, Cano AP, Hartwig J, McCulloch CA, Cybulsky MI
The Journal of cell biology 2012 Apr 2;197(1):115-29
The Journal of cell biology 2012 Apr 2;197(1):115-29
The cell surface proteome of human mesenchymal stromal cells.
Niehage C, Steenblock C, Pursche T, Bornhäuser M, Corbeil D, Hoflack B
PloS one 2011;6(5):e20399
PloS one 2011;6(5):e20399
Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients.
Zhang Y, Li J, Lou J, Zhou Y, Bo L, Zhu J, Zhu K, Wan X, Cai Z, Deng X
Critical care (London, England) 2011;15(1):R70
Critical care (London, England) 2011;15(1):R70
Ro60-associated single-stranded RNA links inflammation with fetal cardiac fibrosis via ligation of TLRs: a novel pathway to autoimmune-associated heart block.
Clancy RM, Alvarez D, Komissarova E, Barrat FJ, Swartz J, Buyon JP
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 15;184(4):2148-55
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 15;184(4):2148-55
C5a enhances dysregulated inflammatory and angiogenic responses to malaria in vitro: potential implications for placental malaria.
Conroy A, Serghides L, Finney C, Owino SO, Kumar S, Gowda DC, Liles WC, Moore JM, Kain KC
PloS one 2009;4(3):e4953
PloS one 2009;4(3):e4953
B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity.
Brudek T, Christensen T, Aagaard L, Petersen T, Hansen HJ, Møller-Larsen A
Retrovirology 2009 Nov 16;6:104
Retrovirology 2009 Nov 16;6:104
Modulation of dendritic cell maturation and function by the Tax protein of human T cell leukemia virus type 1.
Jain P, Ahuja J, Khan ZK, Shimizu S, Meucci O, Jennings SR, Wigdahl B
Journal of leukocyte biology 2007 Jul;82(1):44-56
Journal of leukocyte biology 2007 Jul;82(1):44-56
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- Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD14 APC (purple histogram). Cells in the monocyte gate were used for analysis.
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- Figure 2 Cell surface expression of SAIL in CLL, AML and MM patient samples and normal BMMC and PBMC controls. ( a ) Three CLL specimens analyzed by flow cytometry. CLL cells were identified as CD19/CD5 double-positive cells. The histograms present SAIL (filled) and isotype control (open) staining in the live-cell and the CLL population. ( b ) Flow cytometry analysis of three AML specimens. SAIL expression is assessed in live-cells, CD33-positive and CD34-positive cells. ( c ) Flow cytometry analysis of three MM specimens. CD38 high cells with CD56 expression were gated for MM cells. SAIL expression is assessed in the live-cell and the MM population. ( d and e ) Flow cytometry analysis of SAIL expression in BMMC ( d ) and PBMC ( e ) via co-staining with CD19, CD3, CD14, CD56, CD33, CD34 and a cocktail of lineage (LN) markers. Numbers in histograms are median-fluorescence-intensity fold-change values relative to the isotype control. Three and two representative examples are shown for the tumor and normal samples, respectively.
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- Figure 2 PD-1 and PD-L1 were upregulated on T cells and monocytes in septic shock patients . Blood samples were obtained from 19 septic shock patients and 22 healthy controls and were stained for programmed death-1 (PD-1) and programmed death ligand-1 (PD-L1) gated on CD4 + T cells, CD8 + T cells, and CD14 + monocytes. (a) to (c) Percentage of PD-1 expression on (a) CD4 + T cells and (b) CD8 + T cells, and (c) percentage of PD-L1 expression on CD14 + monocytes. Each dot represents one individual. Data are mean +- standard error of the mean (SEM) of three independent experiments. ** P < 0.01 compared with healthy controls. (d) to (f) Mean fluorescence intensity (relative fluorescence units) of PD-1 expression on (d) CD4 + T cells, (e) PD-1 expression on CD8 + T cells, and (f) PD-L1 expression on CD14 + monocytes Each dot represents one individual. Data are mean +- SEM of three independent experiments. * P < 0.05 compared with healthy controls. (g) Representative PD-1 expression levels on CD4 + T cells and CD8 + T cells, and PD-L1 expression on CD14 + monocytes. Values in the upper-right quadrant indicate the percentage of cells that express PD-1 or PD-L1.
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- Fig 7 Measurement of ROS in CD14 + cells, CD4 + T-cells, and CD8 + T-cells by cellROX stain mean intensity in T2DM patients compared to healthy. CD14 + cells were stained with cellROX green reagent, a marker of ROS, and a CD14 cell marker, CD14-PE. CD14 + -ROX + cells' mean intensity was analyzed by FLOW cytometry. There was an observable increase in ROX mean intensity in CD14 + cells isolated from individuals with T2DM compared to healthy volunteers (Fig. 7A). CD4 + cells were also stained with cellROX green reagent and a CD4 cell marker, CD4-Cy5. CD4 + -ROX + cells' mean intensity was analyzed by FLOW cytometry. There was an observable increase in ROX mean intensity in CD4 + T-cells isolated from individuals with T2DM compared to healthy volunteers (Fig. 7B). CD8 + cells were stained with cellROX green reagent and a CD8 cell marker, CD8-Cy5. CD8 + -ROX + cells' mean intensity was analyzed by FLOW cytometry. There was an observable increase in ROX mean intensity in CD8 + T-cells isolated from individuals with T2DM compared to healthy volunteers (Fig. 7C). Data represents mean +-SE from 5 healthy individuals and 5 individuals with T2DM.
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- FIG. 2. Baseline comparison of the interleukin-6 (IL-6) and reactive oxygen species (ROS) markers between healthy volunteers and HIV-positive individuals. We observed a significant increase in the levels of the proinflammatory cytokine, IL-6 in plasma samples collected from individuals with HIV infection compared to healthy individuals (A) . Data represent mean+-SE from comparing baseline levels of 10 healthy volunteers and 15 HIV-positive individuals, **** P
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- FIG. 6. Difference in plasma IL-6 levels and ROS markers pre- and post-GSH supplementation. Sandwich ELISA was performed to compare the cytokine levels between pre-supplementation (V1) and post-supplementation (V3). Assay of cytokines showed a significant decrease in the levels of IL-6 in plasma samples collected from the lGSH-treatment group. There was no significant difference between the levels of IL-6 from the placebo group when comparing visit 1 and visit 3 (A) . Data represent mean+-SE, * P
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- Figure 1 Effect of OxC-beta on CD14, TLR-4, and TLR-2 levels in vitro . Human THP-1 monocytes (A), fibroblasts (B), and endothelial cells (C), were treated with the indicated concentrations of OxC-beta or vehicle control (DMSO) for 24 hours. Immune receptor content was measured 24 hours post-treatment by FACS analysis. OxC-beta-induced increase in receptor level was assessed relative to untreated control cells using a one-way analysis of variance with Tukey's post test for multiple comparisons. DMSO had no effect on receptor level (result not shown). Phorbol myristate acetate (PMA) was used as a positive control in experiments with THP-1 cells (hatched bars).
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- Figure 5 Determination of activities relative to OxC-beta of (A) OxC-beta polymer and monomer fractions, and (B) oxidized lycopene (OxC-lyc), using a CD14 receptor expression assay. THP-1 cells were treated for 24 hours with the indicated concentrations of compounds. CD14 expression was quantified using FACS analysis. The effect of each compound is shown relative to untreated cells. Points represent the mean and standard error from three separate experiments. (A) Correlation analysis indicates a significant dose effect for each compound on CD14 expression with p-values of 0.0036 for OxC-beta, 0.0034 for the polymer, and 0.0113 for the monomer. Comparison of the relative activity of each compound indicates that the monomer is significantly less active than the polymer (p
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- Figure 1 PMA-induced TM expression mediates morphological changes and differentiation marker expression in THP-1 cells. ( A ) THP-1 cells were treated with 150 nM PMA for 24-72 hours. The total cell lysates were harvested, and the expression of TM was analyzed using western blot analysis. beta-actin was used as a loading control. Five independent experiments have been performed (n = 5) and representative images have been showed. The amount of proteins expression was quantified using densitometry and presented as bar graph. The data are presented as the mean +- SD (n = 5), and * p < 0.05 was considered significant. ( B ) THP-1 cells were transfected with TM siRNA or HA-TM FL plasmid for 24 h followed by PMA stimulation for 72 hours. The morphology of the cells was observed using light microscopy. The adherent differentiated macrophage-like cells are indicated by a white arrowhead. Five independent experiments have been performed (n = 5). The quantification is shown in the right graph. ( C ) The expression of the macrophage cell surface markers CD14 (red) and CD68 (green) was analyzed using immunofluorescence and microscopy. Hoechst staining was used to label the nuclei. The scale bar indicates 100 mum. Five independent experiments have been performed (n = 5), and showed representative images. ( D ) The expression of CD14 and CD68 was analyzed using flow cytometry. Data are expressed as a % of the control, are presented as the mean +- SD and represent the results of three indep
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- Figure 4 TM regulates THP-1 cell differentiation via the PKCdelta-ERK1/2 signaling pathway. ( A ) THP-1 cells were transfected with 2 or 4 mug of PKCalpha, PKCbeta, PKCdelta, PKCepsilon, or PKCtheta shRNA for 24 h. Total cell lysates were purified, and knockdown efficiency was assayed using western blot analysis. ( B ) The THP-1 cells were knocked down by PKCalpha, PKCbeta, PKCdelta, PKCepsilon, and PKCtheta shRNAs for 24 h followed by PMA stimulation for 72 hours. The number of CD14 + cells was scored using flow cytometry. Data are expressed as a % of the control, are presented as the mean +- SD and represent the results of five independent experiments (n = 5, * p < 0.05 was considered significant). ( C ) Different sets of THP-1 cells were transfected with 4 mug of each shRNA for 24 h followed by PMA stimulation for 72 hours. The levels of p21 Cip1/WAF1 and PCNA were analyzed using western blot analysis. ( D ) The THP-1 cells were knocked down by PKCdelta shRNA for 24 h followed by PMA stimulation for 72 hours. The level of PKCdelta and total and phosphorylated ERK1/2 was analyzed using western blot analysis. In western blot analysis, beta-actin and total-ERK1/2 were used as loading controls. The density of each band was quantified using densitometry and related protein expression was presented as bar graph. The data are presented as the mean +- SD, and * p < 0.05 was considered significant (n = 5). ( E ) Lysates of THP-1 cells with PMA stimulation were extracted. Left, immu
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- Fig. 1 Flow cytometry analysis of phenotype characterization of hUCMSCs. Phenotype of CD73, CD90, CD105, CD14, CD34, CD45, CD79a and HLA-DR of hUCMSCs was detected by flow cytometry. Intensity >= 95% represented strong expression while
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- Figure 2 Induced differentiation, flow cytometric and immunosuppressive ability analysis of human umbilical cord mesenchymal stem cells expanded in serum-free medium. After differentiation induction, (a) osteogenesis was confirmed by Alizarin Red (x40), (b) adipogenesis was stained by Oil Red O (x200) and (c) chondrogenesis was analyzed by Toluidine Blue (x100). (d) Serum-free medium (SFM)-expanded human umbilical cord mesenchymal stem cells (hUC-MSCs) at the 10th passage were labeled with antibodies against human antigens CD14-PE, CD19-PE, CD34-FITC, CD45-PE, CD73-PE, CD90-PE, CD105-PE, HLA-ABC-FITC, HLA-DR-PE and Nestin-PE. (e) Expression of hTERT in hUC-MSCs. Graph shows the level of hTERT transcripts of hUC-MSCs cultured in serum-containing medium (SCM) and SFM ( n = 5). Values presented as ratio of positive control (HeLa cells). Immunosuppressive ability of hUC-MSCs was evaluated by co-culturing with human peripheral blood mononuclear cells (hPBMCs). (f) Proliferation of hPBMCs was quantified based the measurement of BrdU incorporation during DNA synthesis. (g) Level of interferon gamma (IFN-gamma) in the supernatant was determined by ELISA.
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- Fig. 2 a Cumulative population-doubling (cPD) levels versus passage number for the four different sources of MSC. Black represents CV-MSC ( n = 7), dark gray UC-MSC ( n = 4), medium gray AT-MSC ( n = 5), and light gray BM-MSC ( n = 6). b IHC-based senescence-associated beta-galactosidase (SA-beta-gal) staining of CV-MSC in early (i, passage 4) and late (ii, passage 9) passages, AT-MSC in passage 6 (iii), BM-MSC in passage 6 (iv), and UC-MSC in passage 2 (v) and passage 4 (vi). Scale = 200 mum. c IHC of CV-MSC (i, ii) and BM-MSC (iii, iv) stained for osteopontin (i, iii) and fibronectin (ii, iv). Scale = 1 mm. d Collagen area (%) after collagen contraction assay for CV-MSC ( n = 4), BM-MSC ( n = 3), UC-MSC ( n = 4), and AT-MSC ( n = 3). Cells in passage 3 were used. Results expressed as mean +- SD, percentage of the total collagen area of the collagen gels without cells. e Surface marker expression of CV-MSC in early passages ( n = 5). Results expressed as mean +- SD (%). f Representative immunofluorescence of early passaged CV-MSC (i, iii) and BM-MSC (iii, iv) stained for SM22alpha (i, iii) and alpha-SMA (ii, iv). Scale = 50 mum. AT adipose tissue, BM bone marrow, CV chorionic villi, MSC mesenchymal stromal cells, UC umbilical cord
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- Fig. 5 KDM6B promotes the differentiation of THP-1 cells (A) THP-1 cells were treated with 100 ng/ml PMA, 2 muM GSK-J4, or DMSO for 48 h. CD14 and CD11b expression levels were confirmed using qRT-PCR and normalized to GAPDH . Results are shown as mean +- SEM, n = 3; *p < 0.05, **p < 0.01. (B) We treated negative control (shNC)- and shKDM6B-transfected THP-1 cells with 100 ng/ml PMA for 48 h. The cells were stained with PE-CD11b and APC-CD14 antibodies. The percentage of cells in each quadrant is indicated in the figure. (C) We treated THP-1 cells with 2 muM GSK-J4 or 0.3 muM alisertib for 48 h. The cells were stained with PE-CD11b and APC-CD14 antibodies. The percentage of cells in each quadrant is indicated in the figure. (D) A model of AURKA regulating KDM6B expression in PMA-mediated THP-1 differentiation.
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- Figure 4. Butyrate upregulates the levels of TLR4 and CD14 on colon cancer cells. The expression levels of TLR4 and CD14 on the membrane of SW480 cells and CT26 cells treated by butyrate and/or LPS were (A) analyzed using a flow cytometer, and (B) the MFI values of TLR4 and CD14 were quantitative analyzed. These experiments were repeated >=3 times, and representative graphs are presented. Compared with the NC group, *P
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- Fig. 1 Responses to Candida spp . in neonatal immune cells. a Phagocytosis of Candida in monocytes (boxes and whiskers), including a representative flow microscopy diagram (white bar = ~10 um). Data pooled from multiple experiments over 14 months (9 to 17 subjects per age group; see Supplemental Data for clinical information on preterm subjects); b IL-1beta and c IL-6 response (blood mononuclear cells) to C. albicans or C. parapsilosis (24 h stimulation; 10 to 18 subjects per age group; boxes and whiskers); ( d ) IL-1beta (24 h; 11 to 21 subjects per age group; boxes and whiskers) and e representative gating for pro-IL-1beta (5 h LPS stimulation), gated on CD14-expressing cells (black = fluorescent-minus one control; orange = unstimulated; blue = LPS; representative preterm sample is from a 26 weeks' infant); f pro-IL-1beta (5 h) or g IL-6 (24 h) in response to LPS, zymosan or curdlan (mononuclear cells; 11 to 21 subjects per age group; boxes and whiskers); for b and c , data was pooled from multiple experiments assayed in four ELISA batches with similar distribution of samples per age group
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- Fig. 5 Gene expression and translation of dectin-1 signaling proteins. a Illustration of selected signaling molecules downstream of dectin-1; b Polysome profiles and c quantification of signalosome genes (qPCR) in monosome, disome, and light and heavy polysome fractions (monocytes). Data are from 4 subjects per age group (boxes and whiskers; RQ = relative quantification); d Quantification of signalosome genes (qPCR) in total RNA fractions (4 to 5 subjects/age group; mean +- SD); e Surface expression of dectin-1 (flow cytometry, mononuclear cells, gated on CD14-expressing cells; data pooled from 10 to 23 subjects per age group; boxes and whiskers); f Representative (cropped) Western blot of MALT1 and Bcl10 protein expression in monocytes after 0 to 60 min LPS stimulation. Representative blot is from a 29 weeks gestation sample. Images cropped from same blot probes with each antibody; cumulative quantification of 4 independent Western blot experiments for g MALT1 and h Bcl10 (mean +- SD)
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- Fig. 8 Inhibition glycolysis results in loss of MALT1 protein expression. Effect of blocking glycolysis (using 2-DG) or of blocking translation (using cycloheximide, as control) on MALT1 protein expression (monocytes). a MALT1 protein was detected by Western blot (left panel; representative of two experiments; cropped images from same blot probed with each antibody) at 8 h and 19 h. Lymphoblastoid cell line (LCL) lysate used as positive control for MALT1 protein expression; MALT1 protein detection ( b ) at 16 h and ( c ) over time (intracellular staining by flow cytometry, gated on CD14-expressing cells; MFI mean fluorescence intensity; dotted line: signal for fluorescence-minus-one staining control MFI level; boxes and whiskers with a paired 2-sided t -test in b ; mean +- SD in c and d ; d corresponding cell viability over time (mean +- SD); 6 subjects. e Effect of MALT1 inhibition on IL-1beta, IL-6, and lactate production at rest and following LPS (mononuclear cells; boxes and whiskers with 2-sided paired t -tests); f correlation between LPS-induced IL-1beta and IL-6, and lactate production (Spearman' r ; * p < 0.05; with dotted regression line); 8 subjects. All experiments were conducted in adult cells