TA321322

antibody from OriGene

Targeting: CHUK IkBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA321322

Provider

OriGene

Product name

Anti-CHUK (Phospho-Thr23) Rabbit Polyclonal Antibody

Antibody type

Polyclonal

Description

Anti-CHUK (Phospho-Thr23) Rabbit Polyclonal Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

CHUK

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

1mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 85 kDa . Positive control: 293 cells treated with serum lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 293 cells untreated with serum lysate Lane2: 293 cells treated with serum lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm; Nucleus. Positive control: Human colon carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human colon carcinoma tissue using CHUK (Phospho-Thr23) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

Validation comment

IHC