NB100-56509AF647

antibody from Novus Biologicals

Targeting: IKBKB IKK-beta, IKK2, IKKB, NFKBIKB

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-56509AF647

Provider

Novus Biologicals

Product name

Mouse Monoclonal IKK beta Antibody

Antibody type

Monoclonal

Description

Protein G purified. An approx. 87 kDa band should be observed.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Red dye

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C in the dark.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: IKK beta Antibody (10AG2) [Alexa Fluor (R) 647] [NB100-56509AF647] - An intracellular stain was performed on HeLa cells with IKK beta antibody (10AG2) NB100-56509 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 ug of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.