PA5-46913

antibody from Invitrogen Antibodies

Targeting: IL23A IL-23, IL-23A, IL23P19, P19, SGRF

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-46913

Provider

Invitrogen Antibodies

Product name

IL-23 p19 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISA, approximately 50% cross-reactivity with recombinant canine IL-23p19, less then 25% cross-reactivity with recombinant feline IL-23p19, less than 5% cross-reactivity with recombinant mouse IL-23p19, recombinant human IL-12p40, and recombinant rat IL-23p40 is observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of a CHO cell line transfected with human IL-23. A PVDF membrane was probed with 1 µg/mL of Goat Anti-human IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46913) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for IL-23 p19 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of IL-23 p19 in a CHO cell line transfected with human IL-23. Samples were incubated in IL-23 p19 polyclonal antibody (Product # PA5-46913) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for IL‚23 p19 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of Mouse IL-23 is shown by decrease in OD (measure of IL-17 secretion) with increasing concentrations of IL-23 polyclonal antibody (PA5-46913).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of IL-23 p19 in mouse splenocytes. Samples were incubated in IL-23 p19 polyclonal antibody (Product # PA5-46913). In the presence of Recombinant Mouse IL‚2 (10 ng/mL), Recombinant Human IL‚23 stimulates IL‚17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL‚17 Quantikine ELISA Kit. Under these conditions, IL‚17 secretion elicited by Recombinant Human IL‚23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‚23 p19 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.2-0.8 µg/mL.