Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 46-7823-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-23 p19 Monoclonal Antibody (23dcdp), PerCP-eFluor™ 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 23dcdp monoclonal antibody reacts with the p19 subunit of human IL-23. This heterodimeric cytokine is composed of two disulfide-linked subunits, p40 and p19. It is closely related to IL-12, with which it shares the p40 subunit. The IL-23 receptor is also heterodimeric and shares the IL-12R\beta1 chain with IL-12, while the IL-23R chain is unique to the IL-23 receptor complex. IL-23R signaling occurs through the Jak/STAT pathway and results in ROR\gammat expression, which promotes maintenance and proliferation of T helper 17 (Th17) cells. Dendritic cells and macrophages produce IL-23 in response to TLR2, TLR4, and TLR8 ligands, as well as agonists of the \beta-glucan receptor, Dectin-1. Our studies suggest donor, kinetics and type of stimulant can result in variation in IL-23 expression levels. Recent publications suggest the p19 subunit may also exist in the absence of association with p40.
- Antibody clone number
- 23dcdp
- Concentration
- 5 µL/Test
Submitted references Prostaglandin E2 inhibits IL-23 and IL-12 production by human monocytes through down-regulation of their common p40 subunit.
Reciprocal regulation of IL-23 and IL-12 following co-activation of Dectin-1 and TLR signaling pathways.
Abundant expression of the interleukin (IL)23 subunit p19, but low levels of bioactive IL23 in the rheumatoid synovium: differential expression and Toll-like receptor-(TLR) dependent regulation of the IL23 subunits, p19 and p40, in rheumatoid arthritis.
Investigating the role of the interleukin-23/-17A axis in rheumatoid arthritis.
How microorganisms tip the balance between interleukin-12 family members.
Kalim KW, Groettrup M
Molecular immunology 2013 Mar;53(3):274-82
Molecular immunology 2013 Mar;53(3):274-82
Reciprocal regulation of IL-23 and IL-12 following co-activation of Dectin-1 and TLR signaling pathways.
Dennehy KM, Willment JA, Williams DL, Brown GD
European journal of immunology 2009 May;39(5):1379-86
European journal of immunology 2009 May;39(5):1379-86
Abundant expression of the interleukin (IL)23 subunit p19, but low levels of bioactive IL23 in the rheumatoid synovium: differential expression and Toll-like receptor-(TLR) dependent regulation of the IL23 subunits, p19 and p40, in rheumatoid arthritis.
Brentano F, Ospelt C, Stanczyk J, Gay RE, Gay S, Kyburz D
Annals of the rheumatic diseases 2009 Jan;68(1):143-50
Annals of the rheumatic diseases 2009 Jan;68(1):143-50
Investigating the role of the interleukin-23/-17A axis in rheumatoid arthritis.
Hillyer P, Larché MJ, Bowman EP, McClanahan TK, de Waal Malefyt R, Schewitz LP, Giddins G, Feldmann M, Kastelein RA, Brennan FM
Rheumatology (Oxford, England) 2009 Dec;48(12):1581-9
Rheumatology (Oxford, England) 2009 Dec;48(12):1581-9
How microorganisms tip the balance between interleukin-12 family members.
Goriely S, Neurath MF, Goldman M
Nature reviews. Immunology 2008 Jan;8(1):81-6
Nature reviews. Immunology 2008 Jan;8(1):81-6
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Normal human peripheral blood cells were stimulated overnight with LPS and Imidazoquinoline (R848) in the presence of Protein Transport Inhibitor Cocktail (Product # 00-4980-03), then intracellularly stained with Anti-Human IL-12/IL-23 p40 PE (Product # 12-7129-82) and Mouse IgG2b K Isotype Control PerCP-eFluor® 710 (Product # 46-4732-82) (left) or Anti-Human IL-23 p19 PerCP-eFluor® 710 (right). Viable cells in the monocyte gate, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), were used for analysis.