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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [3]
- Immunohistochemistry [3]
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- Product number
- PA5-20238 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-23 p19 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is Raji cell lysate.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Raji lysate using a IL-23 polyclonal antibody (Product # PA5-20238) in the (A) absence or (B) presence of blocking peptide at a 1 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human Cell Lines. Loading: 20 µg of lysates per lane. Antibodies: IL-23 p19 Polyclonal Antibody (Product # PA5-20238) (2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human Cell Lines. Loading: 20 µg of lysates per lane. Antibodies: IL-23 p19 Polyclonal Antibody (Product # PA5-20238) (2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human Raji Cells. Loading: 15 µg of lysates per lane. Antibodies: IL-23 p19 Polyclonal Antibody (Product # PA5-20238) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Lane A: Absence of blocking peptide Lane B: Presence of blocking peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IL-23 recombinant protein. Antibodies: IL-23 p19 Polyclonal Antibody (Product # PA5-20238) (4 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Raji cells using a IL-23 polyclonal antibody (Product # PA5-20238) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of Raji cells using IL-23 p19 Polyclonal Antibody (Product # PA5-20238) at 10 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed Raji Cells labeling IL-23 with IL-23 p19 Polyclonal Antibody (Product # PA5-20238) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of Raji cells using a IL-23 polyclonal antibody (Product # PA5-20238) at a 10 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling IL-23 with IL-23 p19 Polyclonal Antibody (Product # PA5-20238) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red) and DAPI staining (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human pancreas tissue using IL-23 p19 Polyclonal Antibody (Product # PA5-20238) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
