MA1-10225

antibody from Invitrogen Antibodies

Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

CD44 protein structure - MA1-10225 shown in red.

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 2 CD44 expressed in the recipient's tissue rather than on the infiltrating-cells enhances the development of T1D in the cell transfer model. A) CD44-deficient non-diabetic NOD male recipients were reconstituted with inflammatory spleen cells derived from WT (CD44 +/+ -CD44 -/- ; marked #1) or CD44-deficient (CD44 -/- -CD44 -/- ; marked #2) diabetic NOD female donors. In addition, WT non-diabetic NOD male recipients were reconstituted with inflammatory spleen cells derived from WT (CD44 +/+ -CD44 +/+ ; marked #3) or CD44-deficient (CD44 -/- -CD44 +/+ ; marked #4) diabetic NOD female donors. Inflammatory cells are marked by arrows and local islet cells by arrowhead. The pancreases of all 4 combinations were removed after detection of diabetes or 200 days after cell transfer. Doing so, pancreases that were harvested approximately at the same time, were stained with anti-CD44 mAb. B) The % of diabetes-free mice (exhibiting less than 250 mg/dL urine glucose) was determined in corresponding four mouse groups, described in A . Statistical analysis by Breslow: #2 versus #3, P < 0.01; #1 versus #2 (follow up 75 to 200 days), P < 0.03; #3 versus #4 (follow up 20 to 75 days), P < 0.03.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4. Mouse IFE. ( A ) Schematics of the IFE (arrow = axis of differentiation). ( B ) t-SNE plot of the IFE cells with the underlying black arrow representing the axis of differentiation. Some incompletely differentiated non IFE B cells lie among the IFE B subpopulation. ( C ) Cell type calling with actual subpopulations in the top color band. ( D ) Presenilin-1 (left panel) and CD44 (middle panel) immunostainings of mouse skin sections. Sections were counterstained with DAPI (epi = epidermis, * = co-localization). ( E ) Number of genes with average expression different from 0 in each IFE layer. ( F ) Number of ligands and receptors involved in LRscore > 0.5 interactions. ( G ) Flow diagram representing the number of interactions between the cell subpopulations. ( H and I ) Chord diagrams of adjacent and remote IFE K2 cell-cell interactions. ( J ) Immune marker gene expression. Il18 is expressed by macrophages, Ifi30 by antigen-presenting cells and Prdm1 by T cells (genecards.org).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Evaluation of the CD marker location in the synovial membrane. Immunohistochemical analysis using DAB (3, 3'-diaminobenzidine, orange/brown staining) was performed to identify the location of CD90, CD44, CD73, CD271, and CD34 expression in the normal (circle) and osteoarthritic (OA, square) synovial membrane. Representative images, containing both fibrous and adipose type synovial membrane, were chosen. The scale bar is set at 50 um. Quantification of the DAB staining was performed with Image ProPlus 6.0 software (Media Cybernetics) in 3 to 6 random regions of interest (ROI) per donor, resulting in a mean percentage of DAB positive staining areas for each donor