H00007185-M01

antibody from Abnova Corporation

Targeting: TRAF1 EBI6

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007185-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007185-M01, RRID:AB_425724

Product name

TRAF1 monoclonal antibody (M01), clone 2G9-G10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant TRAF1.

Antigen sequence

MASSSGSSPRPAPDENEFPFGCPPTVCQDPKEPRA
LCCAGCLSENPRNGEDQICPKCRGEDLQSISPGSR
LRTQEKAHPEVAEAGIGCPFAGVGCSFKGSPQSVQ
EHEVTSQTSHLNLLLGFMKQWKARLGCGLESGPMA
LEQNLSDLQLQAAVEVAGDLEVDCYRAPCSESQEE
LALQHFMKEKLLAELEGKLRVFENIVAVLNKEVEA
SHLALATSIHQSQLDRERILSLEQRVVELQQTLAQ
KDQALGKLEQSLRLMEEASFDGTFLWKITNVTRRC
HESACGRTVSLFSPAFYTAKYGYKLCLRLYLNGDG
TGKRTHLSLFIVIMRGEYDALLPWPFRNKVTFMLL
DQNNREHAIDAFRPDLSSASFQRPQSETNVASGCP
LFFPLSKLQSPKHAYVKDDTMFLKCIVETST


Isotype

IgG

Antibody clone number

2G9-G10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between TRAF2 and TRAF1. HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-TRAF1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)