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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- AP11162PU-N - Provider product page
- Provider
- OriGene
- Product name
- ISG15 (N-term) rabbit polyclonal antibody, Purified
- Antibody type
- Polyclonal
- Description
- ISG15 (N-term) rabbit polyclonal antibody, Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ISG15
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-ISG15 Pab to detect ISG15 in mouse kidney tissue lysate.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of ISG15 (arrow) using Rabbit polyclonal hISG15-A46 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ISG15 gene (Lane 2)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis in 293 cell line lysates (35ug/lane) using ISG15 Antibody (A46) . This demonstrates the ISG15 antibody detected the ISG15 protein (arrow).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using ISG15 Antibody in Jurkat,K562 and AML-2 cell line lysates treated with a small molecular of 0,10,20,30uM for 24 hours. Offered friendly by Biyin Cao, Soochow University, Center for Blood Research.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
