MA5-16009

antibody from Invitrogen Antibodies

Targeting: HIF1A bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8

Antibody data

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Validation data

Product number: MA5-16009 - Provider product page
Provider: Invitrogen Antibodies
Product name: HIF1A Monoclonal Antibody (H1alpha67), DyLight™ 488
Antibody type: Monoclonal
Antigen: Other
Description: In Western blot, multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha.
Conjugate: Green dye
Antibody clone number: H1alpha67
Concentration: 0.7 mg/mL

HIF1A protein structure - MA5-16009 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 Long-term effects of enhanced permeability and hypoxia mediated by deoxygenated PNPs in vivo. a Analysis of Evans blue accumulation in tumours 24 h after the intravenous injection of deoxygenated PNPs. Evans blue in the whole tumour was extracted and quantified by ultraviolet-visible (UV-vis) spectroscopy ( n = 3). b Ex vivo fluorescence images of Evans blue (red) and 4',6-diamidino-2-phenylindole (DAPI) (blue) staining (scale bar, 100 um). c Quantification of Evans blue accumulation in b ( n = 3). d Changes in DO after the intratumoural injection of deoxygenated PNPs and oil nanoparticles (ONPs). e Representative immunofluorescence images of hypoxia-inducible factor-1alpha (HIF-1alpha) (green) staining at 24 h after the intratumoural injection of deoxygenated PNPs and ONPs (scale bar, 100 um). f Quantification of HIF-1alpha expression shown in e ( n = 4). Data are presented as the mean +- s.e.m. * p < 0.05, ** p < 0.01 (unpaired, two-way t tests). Source data are provided as a Source Data file
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 3 Increased tumour permeability and hypoxia mediated by deoxygenated PNPs under laser irradiation. a The accumulation of Evans blue in tumours was quantified 24 h after the intravenous injection of PNPs and 3 h after laser irradiation (5 min, 808 nm, 400 mW cm -2 ), ( n = 3). b Ex vivo fluorescence images of Evans blue (red) in tumour sections from a . Nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI) (blue), (scale bar, 100 um), ( n = 3). c Relative quantification of Evans blue accumulation in b ( n = 4). d Changes in dissolved oxygen (DO) during three cycles of laser irradiation (808 nm, 2 W cm -2 , 20 s) after intratumoural administration. e Changes in DO after laser irradiation (808 nm, 400 mW cm -2 ) for 5 min at 24 h post-intravenous injection ( n = 3). f Western blot of intratumoural hypoxia-inducible factor-1alpha (HIF-1alpha) expression at 24 h after laser irradiation. g , h Tumour sections were stained with anti-pimonidazole antibody (green), anti-HIF-1alpha antibody (green) and DAPI (blue) at 24 h post irradiation (scale bars, 200 um). i Quantification of pimonidazole and HIF-1alpha staining ( n >= 3). Data are presented as the mean +- s.e.m. * p < 0.05, ** p < 0.01 and *** p < 0.001 (unpaired, two-way t tests). Source data are provided as a Source Data file
Conjugate: Green dye