PAB29359

antibody from Abnova Corporation

Targeting: HIF1A bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29359

Provider

Abnova Corporation

Product name

HIF1A polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human HIF1A.

Antigen sequence

KSHPRSPNVLSVALSQRTTVPEEELNPKILALQNA
QRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLS
WKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQS
MDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRA
LD


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with HIF1A polyclonal antibody (Cat # PAB29359) at 1-4 ug/mL concentration shows shows positivity in nucleus and nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil with HIF1A polyclonal antibody (Cat # PAB29359) shows moderate cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)