MA1-81888

antibody from Invitrogen Antibodies

Targeting: NOTCH1 TAN1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-81888

Provider

Invitrogen Antibodies

Product name

NOTCH1 Monoclonal Antibody (mN1A)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Antibody clone number

mN1A

Concentration

1 mg/mL

References

Submitted references

Histone deacetylase 5 promotes the proliferation and invasion of lung cancer cells.

Zhong L, Sun S, Yao S, Han X, Gu M, Shi J
Oncology reports 2018 Oct;40(4):2224-2232

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Characterization of two distinct lymphoproliferative diseases caused by ectopic expression of the Notch ligand DLL4 on T cells.

Xiong H, Maraver A, Latkowski JA, Henderson T, Schlessinger K, Ding Y, Shen J, Tadokoro CE, Lafaille JJ
PloS one 2013;8(12):e84841

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NOTCH 1 was performed by loading 30 µg of HEK293T whole cell lysate per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 1 hour at room temperature. The membrane was probed with a NOTCH 1 monoclonal antibody (Product # MA1-81888) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:40,000 for 1 hour at room temperature. Chemiluminescent detection was performed using ECL substrate. Data courtesy of the Innovators Program.