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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB111 - Provider product page
- Provider
- R&D Systems
- Product name
- Zebrafish BMP-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects zebrafish BMP-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human BMP-2, -3, -3B, -4, -5, -6, -7, -8, or recombinant zebrafish BMP-4 is observed.
- Reactivity
- Zebrafish
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AAI63048- Isotype
- IgG
- Antibody clone number
- 208301
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Alkaline Phosphatase Production Induced by BMP-2a and Neutralization by Zebrafish BMP-2 Antibody. Recombinant Zebrafish BMP-2a (Catalog # 111-BM) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Zebrafish BMP-2a (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Zebrafish BMP-2 Monoclonal Antibody (Catalog # MAB111). The ND50 is typically 5-25 µg/mL in the presence of heparin (2 µg/mL).
