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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- GTX83529 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83529, RRID:AB_10734555
- Product name
- TACC3 antibody [1E11]
- Antibody type
- Monoclonal
- Reactivity
- Human, Rat
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TACC3 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TACC3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TACC3.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-TACC3 mouse monoclonal antibody (GTX83529) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TACC3
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-TACC3 antibody(GTX83529),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either TACC3 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TACC3 antibody (GTX83529), and then analyzed by flow cytometry.
