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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC5875C - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TLR7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TLR7 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Hepatitis C Virus Sensing by Human Trophoblasts Induces Innate Immune Responses and Recruitment of Maternal NK Cells: Potential Implications for Limiting Vertical Transmission.
Giugliano S, Petroff MG, Warren BD, Jasti S, Linscheid C, Ward A, Kramer A, Dobrinskikh E, Sheiko MA, Gale M Jr, Golden-Mason L, Winn VD, Rosen HR
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 15;195(8):3737-47
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 15;195(8):3737-47
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of TLR7 in Ramos Human Cell Line by Flow Cytometry. Ramos human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TLR7 PerCP-conjugated Monoclonal Antibody (Catalog # IC5875C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
