Explore
Validate
Learn
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-56588 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56588, RRID:AB_839014
- Product name
- Rabbit Polyclonal TLR7 Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Myeloid differentiation factor 88 signaling in donor T cells accelerates graft-versus-host disease.
Chromatin remodelling and autocrine TNFα are required for optimal interleukin-6 expression in activated human neutrophils.
Ligation of TLR7 by rheumatoid arthritis synovial fluid single strand RNA induces transcription of TNFα in monocytes.
An imbalance of two functionally and phenotypically different subsets of plasmacytoid dendritic cells characterizes the dysfunctional immune regulation in multiple sclerosis.
Ro60-associated single-stranded RNA links inflammation with fetal cardiac fibrosis via ligation of TLRs: a novel pathway to autoimmune-associated heart block.
Toll-like receptor expression in normal ovary and ovarian tumors.
Role of atopic status in Toll-like receptor (TLR)7- and TLR9-mediated activation of human eosinophils.
Differentially stimulated CD4+ T cells display altered human immunodeficiency virus infection kinetics: implications for the efficacy of antiviral agents.
STAT3- and STAT5-dependent pathways competitively regulate the pan-differentiation of CD34pos cells into tumor-competent dendritic cells.
Pathology of experimental SARS coronavirus infection in cats and ferrets.
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Activation of anti-hepatitis C virus responses via Toll-like receptor 7.
Early onset of inflammation and later involvement of TGFbeta in Duchenne muscular dystrophy.
Matsuoka S, Hashimoto D, Kadowaki M, Ohigashi H, Hayase E, Yokoyama E, Hasegawa Y, Tateno T, Chen X, Aoyama K, Oka H, Onozawa M, Takeda K, Akashi K, Teshima T
Haematologica 2020 Jan;105(1):226-234
Haematologica 2020 Jan;105(1):226-234
Chromatin remodelling and autocrine TNFα are required for optimal interleukin-6 expression in activated human neutrophils.
Zimmermann M, Aguilera FB, Castellucci M, Rossato M, Costa S, Lunardi C, Ostuni R, Girolomoni G, Natoli G, Bazzoni F, Tamassia N, Cassatella MA
Nature communications 2015 Jan 23;6:6061
Nature communications 2015 Jan 23;6:6061
Ligation of TLR7 by rheumatoid arthritis synovial fluid single strand RNA induces transcription of TNFα in monocytes.
Chamberlain ND, Kim SJ, Vila OM, Volin MV, Volkov S, Pope RM, Arami S, Mandelin AM 2nd, Shahrara S
Annals of the rheumatic diseases 2013 Mar;72(3):418-26
Annals of the rheumatic diseases 2013 Mar;72(3):418-26
An imbalance of two functionally and phenotypically different subsets of plasmacytoid dendritic cells characterizes the dysfunctional immune regulation in multiple sclerosis.
Schwab N, Zozulya AL, Kieseier BC, Toyka KV, Wiendl H
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5368-74
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5368-74
Ro60-associated single-stranded RNA links inflammation with fetal cardiac fibrosis via ligation of TLRs: a novel pathway to autoimmune-associated heart block.
Clancy RM, Alvarez D, Komissarova E, Barrat FJ, Swartz J, Buyon JP
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 15;184(4):2148-55
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 15;184(4):2148-55
Toll-like receptor expression in normal ovary and ovarian tumors.
Zhou M, McFarland-Mancini MM, Funk HM, Husseinzadeh N, Mounajjed T, Drew AF
Cancer immunology, immunotherapy : CII 2009 Sep;58(9):1375-85
Cancer immunology, immunotherapy : CII 2009 Sep;58(9):1375-85
Role of atopic status in Toll-like receptor (TLR)7- and TLR9-mediated activation of human eosinophils.
Månsson A, Cardell LO
Journal of leukocyte biology 2009 Apr;85(4):719-27
Journal of leukocyte biology 2009 Apr;85(4):719-27
Differentially stimulated CD4+ T cells display altered human immunodeficiency virus infection kinetics: implications for the efficacy of antiviral agents.
Vatakis DN, Nixon CC, Bristol G, Zack JA
Journal of virology 2009 Apr;83(7):3374-8
Journal of virology 2009 Apr;83(7):3374-8
STAT3- and STAT5-dependent pathways competitively regulate the pan-differentiation of CD34pos cells into tumor-competent dendritic cells.
Cohen PA, Koski GK, Czerniecki BJ, Bunting KD, Fu XY, Wang Z, Zhang WJ, Carter CS, Awad M, Distel CA, Nagem H, Paustian CC, Johnson TD, Tisdale JF, Shu S
Blood 2008 Sep 1;112(5):1832-43
Blood 2008 Sep 1;112(5):1832-43
Pathology of experimental SARS coronavirus infection in cats and ferrets.
van den Brand JM, Haagmans BL, Leijten L, van Riel D, Martina BE, Osterhaus AD, Kuiken T
Veterinary pathology 2008 Jul;45(4):551-62
Veterinary pathology 2008 Jul;45(4):551-62
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Wong CK, Cheung PF, Ip WK, Lam CW
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
Activation of anti-hepatitis C virus responses via Toll-like receptor 7.
Lee J, Wu CC, Lee KJ, Chuang TH, Katakura K, Liu YT, Chan M, Tawatao R, Chung M, Shen C, Cottam HB, Lai MM, Raz E, Carson DA
Proceedings of the National Academy of Sciences of the United States of America 2006 Feb 7;103(6):1828-33
Proceedings of the National Academy of Sciences of the United States of America 2006 Feb 7;103(6):1828-33
Early onset of inflammation and later involvement of TGFbeta in Duchenne muscular dystrophy.
Chen YW, Nagaraju K, Bakay M, McIntyre O, Rawat R, Shi R, Hoffman EP
Neurology 2005 Sep 27;65(6):826-34
Neurology 2005 Sep 27;65(6):826-34
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TLR7 Antibody [NB100-56588] - Analysis of FFPE human brain using TLR7 antibody at 1:20 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Neuronal staining of TLR7 was observed. Staining was performed by Histowiz.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR7 Antibody [NB100-56588] - Intracellular flow analysis of TLR7 in 10^6 ThP1 cells using 2 ug of NB100-56588. Shaded histogram represents cells without antibody. Green: isotype control. Red: TLR7 antibody. Intracellular flow kit was used for this test, and an anti-rabbit IgG PE conjugated secondary.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR7 Antibody [NB100-56588] - Analysis using the PE conjugate of NB100-56588. Staining of TLR7 in human PBMCs (monocytes) using 0.5 ug/10^6 cells of NBP2-24761. The shaded histogram represents cells alone. Green: PE-conjugated rabbit IgG isotype control (20304D). Red: TLR7 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): TLR7 Antibody [NB100-56588] - Analysis using the FITC conjugate of NB100-56588. Staining of TLR7 in TLR7/HEK 293 cells using this antibody at 2 ug/10^6 cells. Green: Vector/HEK 293 cells. Red: TLR7/HEK 293 cells.
