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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB5875 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TLR7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TLR7 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9NYK1- Isotype
- IgG
- Antibody clone number
- 533707
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Satellite glial cells in human trigeminal ganglia have a broad expression of functional Toll-like receptors.
Mitterreiter JG, Ouwendijk WJD, van Velzen M, van Nierop GP, Osterhaus ADME, Verjans GMGM
European journal of immunology 2017 Jul;47(7):1181-1187
European journal of immunology 2017 Jul;47(7):1181-1187
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TLR7 in Ramos Human Cell Line by Flow Cytometry. Ramos human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TLR7 Monoclonal Antibody (Catalog # MAB5875, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.
