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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF-419-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-12 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse IL-12 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing and reducing conditions), this antibody shows less than 10% cross-reactivity with recombinant human IL-12.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Crucial role of OX40/OX40L signaling in a murine model of asthma.
Efficient expression of bioactive murine IL12 as a self-processing P2A polypeptide driven by inflammation-regulated promoters in tumor cell lines.
Viral antigen mediated NKp46 activation of NK cells results in tumor rejection via NK-DC crosstalk.
The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone loss.
Expression of bioactive single-chain murine IL-12 in transgenic plants.
Toll-like receptor (TLR)2 and TLR3 synergy and cross-inhibition in murine myeloid dendritic cells.
Complement receptor 3 blockade promotes IL-12-mediated clearance of Porphyromonas gingivalis and negates its virulence in vivo.
Complement receptor 3 blockade promotes IL-12-mediated clearance of Porphyromonas gingivalis and negates its virulence in vivo.
Lei W, Zeng D, Liu G, Zhu Y, Wang J, Wu H, Jiang J, Huang J
Molecular medicine reports 2018 Mar;17(3):4213-4220
Molecular medicine reports 2018 Mar;17(3):4213-4220
Efficient expression of bioactive murine IL12 as a self-processing P2A polypeptide driven by inflammation-regulated promoters in tumor cell lines.
Lorenzo C, Pérez-Chacón G, Garaulet G, Mallorquín Z, Zapata JM, Rodríguez A
Cancer gene therapy 2015 Nov;22(11):542-51
Cancer gene therapy 2015 Nov;22(11):542-51
Viral antigen mediated NKp46 activation of NK cells results in tumor rejection via NK-DC crosstalk.
Chinnery F, King CA, Elliott T, Bateman AR, James E
Oncoimmunology 2012 Sep 1;1(6):874-883
Oncoimmunology 2012 Sep 1;1(6):874-883
The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone loss.
Liang S, Krauss JL, Domon H, McIntosh ML, Hosur KB, Qu H, Li F, Tzekou A, Lambris JD, Hajishengallis G
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 15;186(2):869-77
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 15;186(2):869-77
Expression of bioactive single-chain murine IL-12 in transgenic plants.
Liu J, Dolan MC, Reidy M, Cramer CL
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2008 Jun;28(6):381-92
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2008 Jun;28(6):381-92
Toll-like receptor (TLR)2 and TLR3 synergy and cross-inhibition in murine myeloid dendritic cells.
Vanhoutte F, Paget C, Breuilh L, Fontaine J, Vendeville C, Goriely S, Ryffel B, Faveeuw C, Trottein F
Immunology letters 2008 Feb 15;116(1):86-94
Immunology letters 2008 Feb 15;116(1):86-94
Complement receptor 3 blockade promotes IL-12-mediated clearance of Porphyromonas gingivalis and negates its virulence in vivo.
Hajishengallis G, Shakhatreh MA, Wang M, Liang S
Journal of immunology (Baltimore, Md. : 1950) 2007 Aug 15;179(4):2359-67
Journal of immunology (Baltimore, Md. : 1950) 2007 Aug 15;179(4):2359-67
Complement receptor 3 blockade promotes IL-12-mediated clearance of Porphyromonas gingivalis and negates its virulence in vivo.
Hajishengallis G, Shakhatreh MA, Wang M, Liang S
Journal of immunology (Baltimore, Md. : 1950) 2007 Aug 15;179(4):2359-67
Journal of immunology (Baltimore, Md. : 1950) 2007 Aug 15;179(4):2359-67
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-12 and Neutralization by Mouse IL-12 Antibody. Recombinant Mouse IL-12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-419-NA). The ND50 is typically 0.01-0.06 µg/mL.
