Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-30202 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBE2L3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLa, JurKat, mouse testis, UBE2L3 shRNA-transfected 293T.
- Concentration
- 0.78 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of UBE2L3 using 50 µg of mouse brain lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a UBE2L3 polyclonal antibody (Product # PA5-30202) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of UBE2L3 was performed by separating 50 µg of mouse tissue extract by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a UBE2L3 Polyclonal Antibody (Product # PA5-30202) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using UBE2L3 Polyclonal Antibody (Product # PA5-30202). Sample (30 µg of whole cell lysate). Lane A: JurKat. Lane B: Hela. 12% SDS PAGE. UBE2L3 Polyclonal Antibody (Product # PA5-30202) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of UBE2L3 in methanol-fixed HeLa cells using a UBE2L3 polyclonal antibody (Product # PA5-30202) at a 1:200 dilution.