GTX48496

antibody from GeneTex

Targeting: A2M CPAMD5, FWP007, S863-7

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

GTX48496

Provider

GeneTex

Proper citation

GeneTex Cat#GTX48496, RRID:AB_11171264

Product name

alpha 2 Macroglobulin antibody

Antibody type

Polyclonal

Antigen

Other

Host

Goat

Isotype

IgG

Vial size

1ml

Storage

Store vial at 4° C for short-term use.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western Blot of Goat anti-Alpha-2-Macroglobulin Antibody (GTX48496). Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ng per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Goat anti Alpha-2-Macroglobulin (GTX48496) was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (on 4 C in blocking buffer). Detection shown was using Dylight 649 conjugated Donkey anti goat (1:10K in TBS/blocking buffer) 1 hr RT.

Validation comment

WB