NB600-939-0.1mg

antibody from Novus Biologicals

Targeting: A2M CPAMD5, FWP007, S863-7

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Antibody Data

Product number

NB600-939-0.1mg

Provider

Novus Biologicals

Product name

Goat Polyclonal alpha 2-Macroglobulin Antibody

Antibody type

Polyclonal

Description

Delipidation and Defibrination.

Reactivity

Human

Host

Goat

Conjugate

Biotin

Isotype

IgG

Vial size

0.1 mg

Concentration

LYOPH

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: alpha 2-Macroglobulin Antibody [Biotin] [NB600-939] - Goat anti Alpha-2-Macroglobulin antibody was used to detect alpha 2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1:10K in TBS 1 hr RT.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: alpha 2-Macroglobulin Antibody [Biotin] [NB600-939] - Lane 1: alpha 2-Macroglobulin Antibody. Lane 2: none. Load: 100 ug per lane. Primary antibody:alpha 2-Macroglobulin biotin conjugated antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.