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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- TA807225 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA807225, RRID:AB_2628370
- Product name
- F12 mouse monoclonal antibody, clone OTI2D12 (formerly 2D12)
- Antibody type
- Monoclonal
- Description
- F12 mouse monoclonal antibody, clone OTI2D12 (formerly 2D12)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- F12
- Isotype
- IgG
- Antibody clone number
- OTI2D12
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY F12 (RC224108, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-F12.(1:2000)
- Validation comment
- WB
