ABIN458009

antibody from antibodies-online

Targeting: F12

No application information provided.

Antibody Data

Product number

ABIN458009

Provider

antibodies-online

Proper citation

Antibodies-Online Cat#ABIN458009, RRID:AB_10784169

Product name

anti-Coagulation Factor XII (Hageman Factor) (F12) antibody

Antibody type

Polyclonal

Antigen

Factor XII (Hageman factor) is a single chain glycoprotein (MW 80,000). It is a serine protease zymogen. FXII is converted to an active enzyme by damage to vascular endothelium. In vitro activation is caused by glass or kaolin. Surface binding promotes activation by kallikrein. FXIIa activates prekallikrein and FXI. Kallikrein activates more FXII. Fragments of FXIIa activate FVII to FVIIa, and plasminogen to plasmin. The complement system is also activated by FXIIa. A link between fibrin clot and the subendothelium is formed by FXIIa cross-linking with fibrin, alpha-2 plasmin inhibitor, fibronectin and Van Willebrand factor. Purified plasma FXII is used as immunogen. Freund€™s complete adjuvant is used in the first step of the immunization procedure.

Reactivity

Human

Host

Goat

Vial size

1 ml

Concentration

Total protein concentration in the antiserum is about 50 mg/ml, the IgG concentration about 10 mg/ml. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:32 as measured against appropriate antigens in precipitin-block titration.

Storage

Lyophilised at +4°C (10 years), reconstituted at or below -20°C (3-5 years), reconstituted at +4°C at least 7 days