Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-34670APC - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal HSP60 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps), more appropriately called as stress response proteins (srps). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone LK1, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Drosophila, Hamster, Porcine, Rabbit, Sheep, Simian, Xenopus
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: HSP60 Antibody (LK1) [Allophycocyanin] [NBP2-34670APC] - An intracellular stain was performed on HeLa cells with HSP60 Antibody (LK1) NBP2-34670APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.