MA3-012-A647

antibody from Invitrogen Antibodies

Targeting: HSPD1 GroEL, HSP60, SPG13

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA3-012-A647

Provider

Invitrogen Antibodies

Product name

HSP60 Monoclonal Antibody (4B9/89), Alexa Fluor™ 647

Antibody type

Monoclonal

Antigen

Other

Description

The MA3012A647 antigen is human placental HSP60. Epitope mapping studies using human HSP60 deletion mutants suggest that this antibody binds either between amino acids 335-366 or 484-547.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Red dye

Isotype

IgG

Antibody clone number

4B9/89

Vial size

50 µL

Concentration

1 mg/mL

Storage

4° C, store in dark, DO NOT FREEZE!

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hsp60 (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Hsp60 Monoclonal Antibody, AlexaFluor 647 conjugate (Product # MA3-012-A647) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Conjugate

Red dye