LS-C355464

antibody from LSBio

Targeting: TF PRO1557, PRO2086

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Radioimmunoassay (Recommended by provider)

Antibody Data

Product number

LS-C355464

Provider

LSBio

Product name

TF / Transferrin Antibody (clone F2H8G6) LS-C355464

Antibody type

Monoclonal

Description

Protein A purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

F2H8G6

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sandwich ELISA of Transferrin was performed by coating wells of a 96-well plate with 100ul of an transferrin chicken polyclonal antibody diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 100ul of StartingBlock T20 (TBS) Blocking Buffer for 2 hours, and 100ul of recombinant transferrin protein ranging from 1.6-1000ng/ml were incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of an Transferrin mouse monoclonal antibody diluted to a concentration of 1 µg/mL was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of a Streptavidin-HRP was incubated for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Sandwich ELISA of Transferrin was performed by coating wells of a 96-well plate with 100ul of an transferrin chicken polyclonal antibody diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 100ul of StartingBlock T20 (TBS) Blocking Buffer for 2 hours, and 100ul of recombinant transferrin protein ranging from 1.6-1000ng/ml were incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of an Transferrin mouse monoclonal antibody diluted to a concentration of 1 µg/mL was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of a Streptavidin-HRP was incubated for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Sandwich ELISA of Transferrin was performed by coating wells of a 96-well plate with 100ul of an transferrin chicken polyclonal antibody diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 100ul of StartingBlock T20 (TBS) Blocking Buffer for 2 hours, and 100ul of recombinant transferrin protein ranging from 1.6-1000ng/ml were incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of an Transferrin mouse monoclonal antibody diluted to a concentration of 1 µg/mL was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of a Streptavidin-HRP was incubated for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.