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LearnMAB7724
antibody from Novus Biologicals
Targeting: AMBP
EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- MAB7724 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal alpha 1-Microglobulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human alpha 1-Microglobulin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat alpha 1-Microglobulin is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references A Retrospective Analysis of the Cartilage Kunitz Protease Inhibitory Proteins Identifies These as Members of the Inter-α-Trypsin Inhibitor Superfamily with Potential Roles in the Protection of the Articulatory Surface.
Smith SM, Melrose J
International journal of molecular sciences 2019 Jan 24;20(3)
International journal of molecular sciences 2019 Jan 24;20(3)
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human alpha 1-Microglobulin by Western Blot. Western blot shows lysates of human liver tissue, Hep3B human hepatocellular carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Huh-7 human hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human alpha 1-Microglobulin Monoclonal Antibody (Catalog # MAB7724) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha 1-Microglobulin at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
