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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- TA500837 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500837, RRID:AB_11128597
- Product name
- Anti-ATP5B mouse monoclonal antibody, clone OTI1B10 (formerly 1B10)
- Antibody type
- Monoclonal
- Description
- Anti-ATP5B mouse monoclonal antibody, clone OTI1B10 (formerly 1B10)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ATP5F1B
- Isotype
- IgG
- Antibody clone number
- OTI1B10
- Vial size
- 100 µl
- Concentration
- 0.71 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (RC201638, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-ATP5B mouse monoclonal antibody (TA500837) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B(RC201638).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500837, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500837, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500837, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-ATP5B antibody(TA500837),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Validation comment
- FC
