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ImmunocytochemistryAntibody data
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- References [2]
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- Western blot [3]
- ELISA [1]
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- Product number
- H00006678-M02 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal SPARC Antibody
- Antibody type
- Monoclonal
- Description
- Ascites. SPARC - secreted protein, acidic, cysteine-rich (osteonectin) (1B2)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.05 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references SPARC expression in patients with high-risk localized soft tissue sarcoma treated on a randomized phase II trial of neo/adjuvant chemotherapy.
The matricellular protein SPARC supports follicular dendritic cell networking toward Th17 responses.
Davis EJ, Zhao L, Lucas DR, Schuetze SM, Baker LH, Zalupski MM, Thomas D, Chugh R
BMC cancer 2016 Aug 20;16:663
BMC cancer 2016 Aug 20;16:663
The matricellular protein SPARC supports follicular dendritic cell networking toward Th17 responses.
Piconese S, Costanza M, Tripodo C, Sangaletti S, Musio S, Pittoni P, Poliani PL, Burocchi A, Passafaro AL, Gorzanelli A, Vitali C, Chiodoni C, Barnaba V, Pedotti R, Colombo MP
Journal of autoimmunity 2011 Dec;37(4):300-10
Journal of autoimmunity 2011 Dec;37(4):300-10
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPARC Antibody (1B2) [H00006678-M02] - Analysis of SPARC expression in transfected 293T cell line by SPARC monoclonal antibody (M02), clone 1B2.Lane 1: SPARC transfected lysate(34.6 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPARC Antibody (1B2) [H00006678-M02] - SPARC monoclonal antibody (M02), clone 1B2. Analysis of SPARC expression in human kidney.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPARC Antibody (1B2) [H00006678-M02] - SPARC monoclonal antibody (M02), clone 1B2 Analysis of SPARC expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: SPARC Antibody (1B2) [H00006678-M02] - Detection limit for recombinant GST tagged SPARC is approximately 3ng/ml as a capture antibody.
