AP02133SU-S

antibody from Acris Antibodies GmbH

Targeting: SPARC BM-40, ON, ONT

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP02133SU-S

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP02133SU-S, RRID:AB_1622576

Product name

anti SPARC / Osteonectin

Antibody type

Polyclonal

Antigen

Recombinant Human Osteonectin

Reactivity

Human

Host

Rabbit

Vial size

20 µl

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blot analysis of Osteonectin isolated from Human bone. Osteonectin was treated with N-glucosidase F or endoglycosidase H, separated by SDS-PAGE and immunobotted with AP02133SU at 1/2000 dilution. This antibody detects glycosylated Osteonectin. Kaufmann B et al. (2004) Glycobiology 7: 609-619.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP02133SU Osteonectin antibody staining of Paraffin Section of fetal calvaria. The bone tissue was decalcified in 10% formic acid. The section was incubated with AP02133SU overnight and detected using ABC vectastain kit. DAB was used as the chromogen. The sections were counterstained with Mayer's hematoxylin.