Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-66381-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Butyrylcholinesterase/BCHE Antibody
- Antibody type
- Monoclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Canine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Butyrylcholinesterase/BCHE Antibody (OTI1E1) [NBP2-66381] - Analysis of extracts (35ug) from 9 different cell lines by using anti-BCHE monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Butyrylcholinesterase/BCHE Antibody (OTI1E1) [NBP2-66381] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCHE (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCHE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Butyrylcholinesterase/BCHE Antibody (OTI1E1) [NBP2-66381] - HEK293T cells transfected with either RC202198 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BCHE antibody, and then analyzed by flow cytometry.