Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 44-1078G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Vinculin (Tyr1065) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references FAK Shutdown: Consequences on Epithelial Morphogenesis and Biomarker Expression Involving an Innovative Biomaterial for Tissue Regeneration.
The proprotein convertase furin inhibits IL-13-induced inflammation in airway smooth muscle by regulating integrin-associated signaling complexes.
S100A4 is activated by RhoA and catalyses the polymerization of non-muscle myosin, adhesion complex assembly and contraction in airway smooth muscle.
The Role of Hypoxia in Corneal Extracellular Matrix Deposition and Cell Motility.
Inhibition of Smooth Muscle Cell Proliferation and Migration by a Talin Modulator Attenuates Neointimal Formation after Femoral Arterial Injury.
SHP2-Independent Tyrosine Dephosphorylation of Cortactin and Vinculin during Infection with Helicobacter pylori.
Paxillin-Y118 phosphorylation contributes to the control of Src-induced anchorage-independent growth by FAK and adhesion.
The Helicobacter pylori CagA protein disrupts matrix adhesion of gastric epithelial cells by dephosphorylation of vinculin.
Wang X, Steinberg T, Dieterle MP, Ramminger I, Husari A, Tomakidi P
International journal of molecular sciences 2021 Sep 10;22(18)
International journal of molecular sciences 2021 Sep 10;22(18)
The proprotein convertase furin inhibits IL-13-induced inflammation in airway smooth muscle by regulating integrin-associated signaling complexes.
Wu Y, Huang Y, Zhang W, Gunst SJ
American journal of physiology. Lung cellular and molecular physiology 2021 Jul 1;321(1):L102-L115
American journal of physiology. Lung cellular and molecular physiology 2021 Jul 1;321(1):L102-L115
S100A4 is activated by RhoA and catalyses the polymerization of non-muscle myosin, adhesion complex assembly and contraction in airway smooth muscle.
Zhang W, Gunst SJ
The Journal of physiology 2020 Oct;598(20):4573-4590
The Journal of physiology 2020 Oct;598(20):4573-4590
The Role of Hypoxia in Corneal Extracellular Matrix Deposition and Cell Motility.
Onochie OE, Onyejose AJ, Rich CB, Trinkaus-Randall V
Anatomical record (Hoboken, N.J. : 2007) 2020 Jun;303(6):1703-1716
Anatomical record (Hoboken, N.J. : 2007) 2020 Jun;303(6):1703-1716
Inhibition of Smooth Muscle Cell Proliferation and Migration by a Talin Modulator Attenuates Neointimal Formation after Femoral Arterial Injury.
Lim IR, Kim C, Jung JW, Kim JH, Hong SJ
Korean circulation journal 2020 Jul;50(7):613-624
Korean circulation journal 2020 Jul;50(7):613-624
SHP2-Independent Tyrosine Dephosphorylation of Cortactin and Vinculin during Infection with Helicobacter pylori.
Knorr J, Backert S, Tegtmeyer N
European journal of microbiology & immunology 2020 Apr 7;10(1):20-27
European journal of microbiology & immunology 2020 Apr 7;10(1):20-27
Paxillin-Y118 phosphorylation contributes to the control of Src-induced anchorage-independent growth by FAK and adhesion.
Sachdev S, Bu Y, Gelman IH
BMC cancer 2009 Jan 12;9:12
BMC cancer 2009 Jan 12;9:12
The Helicobacter pylori CagA protein disrupts matrix adhesion of gastric epithelial cells by dephosphorylation of vinculin.
Moese S, Selbach M, Brinkmann V, Karlas A, Haimovich B, Backert S, Meyer TF
Cellular microbiology 2007 May;9(5):1148-61
Cellular microbiology 2007 May;9(5):1148-61
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Peptide CompetitionLysates were prepared from COS cells co-transfected withactivated Src and His-tagged chicken vinculin cDNA whichwere either untreated (1 and 6) or treated with vanadate for 24hr (2-5 and 7-10).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Peptide CompetitionLysates were prepared from COS cells co-transfected withactivated Src and His-tagged chicken vinculin cDNA whichwere either untreated (1 and 6) or treated with vanadate for 24hr (2-5 and 7-10).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-Vinculin pTyr1065 Antibody was done on 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Vinculin pTyr1065 Antibody (Product # 44-1078G) at 1:250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing membranous localization. Panel e is a no primary antibody control. The images were captured at 20X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 FAK and adhesion modulate v-Src-induced phosphorylation of various Src substrates . (A) Lysates from adherent cultures of FAK+/+[puro], FAK-/-[puro], FAK+/+[v-Src] and FAK-/-[v-Src] cells were probed either directly by IB for specific phosphorylated form(s) of the Src substrate proteins, total substrate protein levels or GAPDH, or probed for total phosphorylated protein by immunoprecipitating with substrate-specific Ab followed by IB for phosphotyrosine using MAb4G10. (B) Same IB or IP/IB analysis as in panel A using lysates of suspension cultures. Each of these blots is typical of at least duplicate independent experiments.