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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- MA5-24598 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fibulin 1 Monoclonal Antibody (CL0337)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: CESGIHNCLP DFICQNTLGS FRCRPKLQCK SGFIQDALGN CIDINECLSI SAPCPIGHTC INTEGSYTCQ KNVPNCGRGY HLNEEGTRCV DVDECAPPAE PCGKGHRCVN SPGSFRCECK TGYYFDGISR MCVDVNE
- Antibody clone number
- CL0337
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Fibulin 1 in Lane1: Marker (kDa), Lane 2: Human plasma. Samples were probed using a Fibulin 1 Monoclonal Antibody (Product # MA5-24598).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Fibulin 1 Monoclonal Antibody (CL0337) (Product # MA5-24598). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Fibulin 1 in MCF7 cells using the Fibulin 1 Monoclonal Antibody (CL0337) (Product # MA5-24598), showing no specific staining of vesicles. MCF7 cells serves as a negative control based on RNA-seq values. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Fibulin 1 in human cervix, uterine and liver tissues using Fibulin 1 Monoclonal Antibody (CL0337) (Product # MA5-24598). Corresponding FBLN1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Fibulin 1 in human uterine cervix using Fibulin 1 Monoclonal Antibody (CL0337) (Product # MA5-24598) shows strong positivity in the extracellular matrix.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Fibulin 1 in human prostate using Fibulin 1 Monoclonal Antibody (CL0337) (Product # MA5-24598) shows moderate positivity in the extracellular matrix.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Fibulin 1 in human breast cancer using Fibulin 1 Monoclonal Antibody (CL0337) (Product # MA5-24598) shows moderate positivity in the extracellular matrix of tumor stroma.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Fibulin 1 in human liver using Fibulin 1 Monoclonal Antibody (CL0337) (Product # MA5-24598) shows no positivity as expected.
