Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [8]
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Validation data
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- Product number
- LS-B14621 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ SP1 Antibody (aa735-785) LS-B14621
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Storage
- Store at 2-8°C for up to one year.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human Sp1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. Antibodies: Affinity purified goat anti-Sp1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Mouse Sp1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, Renca, and TCMK-1 cells. Antibodies: Affinity purified goat anti-Sp1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of human RRP9 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-RRP9 antibody used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of Human Sp1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. Antibodies: Affinity purified goat anti-Sp1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of Mouse Sp1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, Renca, and TCMK-1 cells. Antibodies: Affinity purified goat anti-Sp1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of human RRP9 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-RRP9 antibody used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of Human Sp1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. Antibodies: Affinity purified goat anti-Sp1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of Mouse Sp1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, Renca, and TCMK-1 cells. Antibodies: Affinity purified goat anti-Sp1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.