Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- H00051176-M05 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00051176-M05, RRID:AB_2249878
- Product name
- Mouse Monoclonal LEF1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. LEF1 (1H2)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LEF1 Antibody (1H2) [H00051176-M05] - Analysis of LEF1 expression in transfected 293T cell line by LEF1 monoclonal antibody (M05), clone 1H2. Lane 1: LEF1 transfected lysatE (44 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LEF1 Antibody (1H2) [H00051176-M05] - Analysis of LEF1 expression in Jurkat (Cat # L017V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: LEF1 Antibody (1H2) [H00051176-M05] - Detection limit for recombinant GST tagged LEF1 is approximately 0.3ng/ml as a capture antibody.