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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MA5-14966 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LEF1 Monoclonal Antibody (E.281.8)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- E.281.8
- Vial size
- 100 µL
- Concentration
- 157 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout of LEF1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR660502_LV and CRISPR660521_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of LEF1 was performed by loading 30 µg of Jurkat wild type (Lane 1) and Jurkat LEF1 KO (Lane 2) modified whole cell extracts. The blot was probed with Anti-LEF1 Monoclonal Antibody (E.281.8)(Product # MA5-14966) using 1:1000 dilution and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to LEF1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on nuclear enriched cell extracts (30 µg lysate) of HT-29 (Lane 1), Molt-4 (Lane 2), Jurkat (Lane 3), Caco-2 (Lane 4) and tissue extract of Mouse Colon (Lane 5). The blot was probed with anti-LEF1 Antibody (Product # MA5-14966, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 41 kDa band corresponding to LEF1 was observed across all the cell lines except Caco-2 and Mouse Colon tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of LEF1 in HCT-15 cells using a LEF1 monoclonal antibody (Product # MA5-14966) (green) and a Pan-Keratin monoclonal antibody (red).
